NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE135478 Query DataSets for GSE135478
Status Public on Oct 17, 2019
Title PTENa/b paradoxically promote carcinogenesis through WDR5-H3K4me3 axis [ChIP-seq 2]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report that USP9X and FBXW11 selectively regulate the stability of PTENa/b but not PTEN proteins by deubiquitination and ubiquitination respectively. USP9X promotes and FBXW11 suppresses tumorigenesis mediated by PTENa/b. In contrast to the current paradigm for PTEN as a tumor suppressor, PTENa/b promote tumorigenesis of cancer cells in a phosphatase-independent manner. Mechanistically, PTENa/b localized in the nucleus regulate expressions of tumor-promoting genes such as NOTCH3 in the similar way as the H3K4 presenter WDR5. Further, PTENa/b but not PTEN directly interact with WDR5 to promote trimethylation of H3K4 and maintain a tumor-promoting signature.
 
Overall design To investigate the influence of H3K4me3 at gene promoters with or without PTENa/b depletion by ChIP seq in SMMC-7721.
 
Contributor(s) Shen SM, Zhang C, Ge MK, Dong SS
Citation(s) 31685992
Submission date Aug 06, 2019
Last update date Jan 16, 2020
Contact name ge mengkai
E-mail(s) gemengk@sjtu.edu.cn
Phone 15201867201
Organization name Shanghai Jiao Tong University School of Medicine (SJTU-SM)
Street address shanghai
City shanghai
State/province State...
ZIP/Postal code 13185541251gmk
Country China
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM4011081 ChIP-gNS_FRRC190665538-1a
GSM4011082 ChIP-gPTENL_FRRC190665539-1a
GSM4011083 Input-gNS_FRRC190665536-1a
This SubSeries is part of SuperSeries:
GSE126984 PTEN╬▒/╬▓ paradoxically promote carcinogenesis through WDR5-H3K4me3 axis
Relations
BioProject PRJNA559009
SRA SRP217603

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135478_gNS-CHIP_vs_INPUT.bw 232.8 Mb (ftp)(http) BW
GSE135478_gPTENL-CHIP_vs_INPUT.bw 256.3 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap