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| Status |
Public on Apr 20, 2020 |
| Title |
RNA sequencing of CD4+ and CD8+ subsets of human engineered regulatory T cell lines expressing FOXP3, FOXP+Helios-FL, FOXP3+Helios-Δ3b |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report RNA sequencing data for three human engineered regulatory T cell lines expressing FOXP3, FOXP+Helios-FL, FOXP3+Helios-Δ3b. From total RNA from 3 biological replicates from each cell line, we generated RNA Sequencing data that compared gene expression between CD4+ and CD8+ cells for each cell line. RNA Sequencing demonstrated that the addition of full-length Helios changed gene expression in cellular pathways and the Treg signature compared to FOXP3 alone or the other major Helios isoform.
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| |
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| Overall design |
Examination of CD4+ and CD8+ cells from three human engineered regulatory T cells cell lines.
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| |
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| Contributor(s) |
Markiewicz MA |
| Citation(s) |
32259202 |
| |
| Submission date |
Aug 06, 2019 |
| Last update date |
Apr 20, 2020 |
| Contact name |
Mary A Markiewicz |
| E-mail(s) |
mmarkiewicz@kumc.edu
|
| Phone |
913-588-7151
|
| Organization name |
University of Kansas Medical Center
|
| Department |
Microbiology, Molecular Genetics & Immunology
|
| Lab |
Mary Markiewicz
|
| Street address |
3901 Rainbow Blvd. MS 3029
|
| City |
Kansas City |
| State/province |
KS |
| ZIP/Postal code |
66160 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (18)
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| Relations |
| BioProject |
PRJNA558955 |
| SRA |
SRP217539 |
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