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Series GSE135452 Query DataSets for GSE135452
Status Public on Apr 20, 2020
Title RNA sequencing of CD4+ and CD8+ subsets of human engineered regulatory T cell lines expressing FOXP3, FOXP+Helios-FL, FOXP3+Helios-Δ3b
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We report RNA sequencing data for three human engineered regulatory T cell lines expressing FOXP3, FOXP+Helios-FL, FOXP3+Helios-Δ3b. From total RNA from 3 biological replicates from each cell line, we generated RNA Sequencing data that compared gene expression between CD4+ and CD8+ cells for each cell line. RNA Sequencing demonstrated that the addition of full-length Helios changed gene expression in cellular pathways and the Treg signature compared to FOXP3 alone or the other major Helios isoform.
Overall design Examination of CD4+ and CD8+ cells from three human engineered regulatory T cells cell lines.
Contributor(s) Markiewicz MA
Citation(s) 32259202
Submission date Aug 06, 2019
Last update date Apr 20, 2020
Contact name Mary A Markiewicz
Phone 913-588-7151
Organization name University of Kansas Medical Center
Department Microbiology, Molecular Genetics & Immunology
Lab Mary Markiewicz
Street address 3901 Rainbow Blvd. MS 3029
City Kansas City
State/province KS
ZIP/Postal code 66160
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (18)
GSM4010380 1-2_CD4
GSM4010381 1-3_CD4
GSM4010382 1-4_CD4
BioProject PRJNA558955
SRA SRP217539

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135452_FOXP3_CD4_cpm.txt.gz 374.7 Kb (ftp)(http) TXT
GSE135452_FOXP3_CD8_cpm.txt.gz 372.5 Kb (ftp)(http) TXT
GSE135452_FOXP3_Helios-FL_CD4_cpm.txt.gz 375.4 Kb (ftp)(http) TXT
GSE135452_FOXP3_Helios-FL_CD8_cpm.txt.gz 376.1 Kb (ftp)(http) TXT
GSE135452_FOXP3_Helios-d3B_CD4_cpm.txt.gz 376.0 Kb (ftp)(http) TXT
GSE135452_FOXP3_Helios-d3B_CD8_cpm.txt.gz 377.5 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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