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| Status |
Public on May 18, 2021 |
| Title |
SS18 is a phase-separation protein relocating from pluripotent to somatic loci during mESC differentiation [ChIP-Seq Dataset 1] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The transition from pluripotent to somatic states marks a critical checkpoint in mammalian development. How this is regulated remains largely unresolved. Here we report the identification of SS18 by whole genome CRISPR screen as a checkpoint regulator. SS18 regulates pluripotent to somatic transition or PST by relocating from pluripotent loci to somatic ones accompanied by corresponding chromatin closing-opening and remodeling of SS18-centric protein complexes. Mechanistically, SS18 forms phase separation granules (PSG) with an intrinsically disordered region (IDR) rich in tyrosine (Y), which, once mutated, no longer form PSG nor rescue SS18-/- defect in PST. These results reveal that rapid cell fate decision can be achieved by massive redistribution of chromatin remodeling37 complexes through phase separation.
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| Overall design |
We analyzed H3K27ac binding during differentiation and the dynamic changes of binding sites of Ss18 in the process of differentiation triggered by JUN in mESCs, EB and EpiSCs.
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| Contributor(s) |
Kuang J, Pei D |
| Citation(s) |
34215745, 35710570 |
| Submission date |
Aug 05, 2019 |
| Last update date |
Jun 27, 2022 |
| Contact name |
kuang junqi |
| E-mail(s) |
kuangjunqi@westlake.edu.cn
|
| Organization name |
westlake University
|
| Street address |
No.18 Shilongshan Road
|
| City |
hangzhou |
| ZIP/Postal code |
310024 |
| Country |
China |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE135451 |
SS18 is a phase-separation protein relocating from pluripotent to somatic loci during mESC differentiation |
|
| Relations |
| BioProject |
PRJNA558754 |
| SRA |
SRP217498 |
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