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Series GSE135396 Query DataSets for GSE135396
Status Public on May 18, 2021
Title SS18 is a phase-separation protein relocating from pluripotent to somatic loci during mESC differentiation [ChIP-Seq Dataset 1]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The transition from pluripotent to somatic states marks a critical checkpoint in mammalian development. How this is regulated remains largely unresolved. Here we report the identification of SS18 by whole genome CRISPR screen as a checkpoint regulator. SS18 regulates pluripotent to somatic transition or PST by relocating from pluripotent loci to somatic ones accompanied by corresponding chromatin closing-opening and remodeling of SS18-centric protein complexes. Mechanistically, SS18 forms phase separation granules (PSG) with an intrinsically disordered region (IDR) rich in tyrosine (Y), which, once mutated, no longer form PSG nor rescue SS18-/- defect in PST. These results reveal that rapid cell fate decision can be achieved by massive redistribution of chromatin remodeling37 complexes through phase separation.
Overall design We analyzed H3K27ac binding during differentiation and the dynamic changes of binding sites of Ss18 in the process of differentiation triggered by JUN in mESCs, EB and EpiSCs.
Contributor(s) Kuang J, Pei D
Citation(s) 34215745, 35710570
Submission date Aug 05, 2019
Last update date Jun 27, 2022
Contact name kuang junqi
Organization name westlake University
Street address No.18 Shilongshan Road
City hangzhou
ZIP/Postal code 310024
Country China
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (9)
GSM4007594 EB, Ss18-ChIP-seq
GSM4007595 EpiSCs, Ss18-ChIP-seq
GSM4007596 ESCs, H3K27ac-ChIP-seq.0h
This SubSeries is part of SuperSeries:
GSE135451 SS18 is a phase-separation protein relocating from pluripotent to somatic loci during mESC differentiation
BioProject PRJNA558754
SRA SRP217498

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Supplementary file Size Download File type/resource
GSE135396_RAW.tar 1.2 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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