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Series GSE135362 Query DataSets for GSE135362
Status Public on May 29, 2020
Title Compared gene expression profile of Lgr5-expressing and deficient stem cells in the intestine
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The Lgr5 receptor is a marker of intestinal stem cells (ISCs) that regulates Wnt/b-catenin signaling. In this study, phenotype analysis of knockin/knockout Lgr5-eGFP-IRES-Cre and Lgr5-DTReGFP embryos revealed that Lgr5 deficiency during Wnt-mediated cytodifferentiation results in amplification of ISCs and early differentiation into Paneth cells, which can be counteracted by in utero treatment with the Wnt inhibitor LGK974. Conditional ablation of Lgr5 postnatally, but not in adults, altered stem cell fate towards the Paneth lineage. Together, these in vivo studies suggest that Lgr5 is part of a feedback loop to adjust the Wnt tone in ISCs. Moreover, transcriptome analyses revealed that Lgr5 controls fetal ISC maturation associated with acquisition of a definitive stable epithelial phenotype, that depends on the capacity of ISCs to generate their own extracellular matrix. Finally, using the ex vivo culture system, evidences are provided that Lgr5/Rspondin 2 interaction negatively regulates the pool of ISCs in organoids, revealing a sophisticated regulatory process for Wnt signaling in ISC.
 
Overall design mRNA profiles of knockin/knockout Lgr5-eGFP-IRES-Cre and Lgr5-DTReGFP embryos versus heterozygous ISC at embryonic and adult stage.
Web link https://www.embopress.org/doi/full/10.15252/embr.201949224
 
Contributor(s) Fernandez Vallone V, Leprovots M, Ribatallada-Soriano D, Gerbier R, Lefort A, Libert F, Vassart G, Garcia M
Citation(s) 32468660
Submission date Aug 04, 2019
Last update date Jun 01, 2020
Contact name Frederick Libert
E-mail(s) flibert@ulb.ac.be
Organization name ULB
Department IRIBHM
Lab C2-140
Street address 808 route de Lennik
City Brussels
ZIP/Postal code B-1070
Country Belgium
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (45)
GSM4006909 Embryonic Lgr5-DTReGFP heterozygous intestinal SC pool 1
GSM4006910 Embryonic Lgr5-DTReGFP (null) homozygous intestinal SC pool 1
GSM4006911 Embryonic Lgr5-DTReGFP heterozygous intestinal SC pool 2
Relations
BioProject PRJNA558562
SRA SRP217338

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Supplementary file Size Download File type/resource
GSE135362_CPM_of_intestinal_stem_cells_expressing_or_not_Lgr5.txt.gz 1.1 Mb (ftp)(http) TXT
GSE135362_Lgr5-expressing_vs_KO_Intestinal_stem_cells.txt.gz 1.8 Mb (ftp)(http) TXT
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Processed data are available on Series record
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