|
| Status |
Public on Aug 01, 2020 |
| Title |
DOT1L-mediated Murine Neuronal Differentiation associates with H3K79me2 Accumulation and preserves SOX2-Enhancer Accessibility |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To study whether acute inhibition of DOT1L induces global chromatin states alterations, we profile and compare the transcriptome, chromatin accessibility and epigenome (H3K4me3, H3K4me1, H3K27ac, H3K27me3, H3K36me3, H3K9me3, H3K79me2) of mESC and ES-derived NPC, treated with DMSO or EPZ5676.
|
| |
|
| Overall design |
full epigenome (duplicates), transcriptome (triplicates) and chromatin acceccibility profiling (duplicates) of mouse embryonic stem cells and in-vitro differentiated neurons, treated with DMSO and EPZ5676
|
| |
|
| Contributor(s) |
Ferrari F, Arrigoni L, Trompouki E, Vogel T, Manke T |
| Citation(s) |
33060580 |
| Submission date |
Aug 02, 2019 |
| Last update date |
Nov 02, 2020 |
| Contact name |
Thomas Manke |
| E-mail(s) |
manke@ie-freiburg.mpg.de
|
| Organization name |
Max Planck Institute of Immunobiology and Epigenetics
|
| Street address |
Stuebeweg 51
|
| City |
Freiburg |
| ZIP/Postal code |
79108 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (86)
|
|
| Relations |
| BioProject |
PRJNA558383 |
| SRA |
SRP217223 |
Less...
More...