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Series GSE135310 Query DataSets for GSE135310
Status Public on Aug 18, 2020
Title Time series single cell transcriptomics of cardiac inflammation after myocardial infarction in mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We performed time-series single-cell RNA-seq analysis of blood CD11b+ cells and of the inflammatory infiltrate in the heart of C57BL/6J male mice in a model of permanent myocardial infarction.
Overall design Cardiac CD45+ total leukocytes (datasets F8, F9, F11, F12, G1), cardiac CD11b+ myeloid cells (dataset H10), or blood and cardiac CD11b+ cells (Sample C11_2) were extracted from the blood or heart of mice in the steady state and at various time points after myocardial infarction or sham surgery in a murine model of permanent myocardial ischemia induced by ligation of the left descendant coronary artery, and analyzed by single-cell RNA-sequencing. In the H10 dataset, CD11b+ cells were sampled at 1, 3, and 5 days after infarction, labeled with CITE-Seq and hashtag antibodies and pooled for multiplexed analysis. In Sample C11_2, blood and cardiac CD11b+ cells from control animals and mice at 1 and 3 days after sham or myocardial infarction surgery were labeled with CITE-seq and hashtag antibodies and pooled for multiplexed analysis.
Contributor(s) Vafadarnejad E, Rizzo G, Krampert L, Arampatzi P, Nugroho VA, Schulz D, Roesch M, Zernecke A, Saliba AE, Cochain C
Citation(s) 32811295
Submission date Aug 02, 2019
Last update date Jun 17, 2022
Contact name Antoine-Emmanuel Saliba
Phone +49-931-31-81341
Organization name Helmholtz Institute for RNA-based Infection Research
Street address Josef-Schneider-Straße 2 / D15
City Würzburg
ZIP/Postal code 97080
Country Germany
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (7)
GSM4005123 F8
GSM4005124 F9
GSM4005125 F11
BioProject PRJNA558375
SRA SRP217210

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135310_RAW.tar 2.1 Gb (http)(custom) TAR (of H5, MTX, TSV, TXT)
GSE135310_README.txt 420 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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