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Series GSE135290 Query DataSets for GSE135290
Status Public on Aug 01, 2020
Title Streamlined low-input transcriptomics through EASY-RNAseq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary we describe a streamlined RNAseq protocol (EASY RNAseq) for sensitive transcriptome assessment starting from low amount of input materials. EASY RNAseq is technically robust enough for sequencing small pools of homogenous and heterogeneous cells, recovering higher numbers of genes and with a more even expression distribution pattern than other commonly used methods.
Overall design To evaluate the capbility of EASY-RNASeq respect to the amount of input materials, different amount of input materials extracted from mice spleen(0.1ng, 0.5ng, 1.0ng, 5.0ng) were used for constructing EASY-RNASeq libraries. And for cells sample, different number of input cells obtained from murine CD3+ T cells(1e2, 5e2, 1e3, 1e4) were used to do EASY-RNASeq library construction. All libraries were sequenced by Illumina platform.
Contributor(s) Zhou Y, Xu H
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BioProject PRJNA542941
Submission date Aug 02, 2019
Last update date Aug 01, 2020
Contact name peng zhou
Organization name First Affiliated Hospital of Army Medical University
Street address 重庆市大渡口区天安数码城5栋10层
City chongqing
ZIP/Postal code 400000
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (23)
GSM4002782 100cells input rep1
GSM4002783 100cells input rep2
GSM4002784 100cells input rep3
SRA SRP198420

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135290_all_feature.txt.gz 650.7 Kb (ftp)(http) TXT
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Processed data are available on Series record

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