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Series GSE135187 Query DataSets for GSE135187
Status Public on Jul 16, 2021
Title RNA profiling of human lymphatic endothelial cells exposed to melanoma exosomes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary In order to study the influence of melanoma-derived exosomes in the lymphatic vasculature within the lymph nodes, we have profile the transcriptional changes occurring in human lymphatic endothelial cells treated with melanoma-secreted exosomes or with the conditioned medium from melanoma cells depleted of exosomes.
Overall design Human lymphatic endothelial cells (HMVECs) were treated for 24h and 48h with exosomes from SK-MEL-147 melanoma cell line and conditioned medium from the same line depleted of exosomes. RNA was extracted with trizol and subsequent Qiagen columns. RNA sequencing was performed afterwards.
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Contributor(s) Silva SG, Graña O, Peinado H
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Submission date Jul 31, 2019
Last update date Dec 14, 2021
Contact name Osvaldo Graña
Organization name CNIO
Department Structural Biology
Lab Bioinformatics Unit
Street address C/ Melchor Fernández Almagro, 3
City Madrid
State/province Comunidad de Madrid
ZIP/Postal code 28029
Country Spain
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (15)
GSM3993139 EXO_10
GSM3993140 EXO_11
GSM3993141 EXO_12
BioProject PRJNA557684
SRA SRP216946

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135187_cuffnorm_FPKMs.txt.gz 1.0 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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