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Series GSE135121 Query DataSets for GSE135121
Status Public on Jul 28, 2021
Title Identification and characterization analysis of the different genes in primary neurons response to circSCMH1 using RNA-seq.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Whole RNA-sequencing analysis was performed by LC-Bio. Day-10 neurons from the Vector or circSCMH1 overexpression groups were collected in Trizol. Total RNAs were extracted from Trizol. UMI technology was used to label each sequence fragment with sequence tags, which minimizes the interference of duplication generated by PCR amplification on the quantitative accuracy of transcriptome. RNA-seq reads were aligned to the mouse genome (GRCh37/hg19) using the software Hisat2 (2.0.4). Transcript abundance was quantified as fragments per kilo base of exon per million fragments mapped (FPKM). Differentially expressed genes were determined by DESeq2 (DOI: 10.1186/s13059-014-0550-8).
Overall design Examination of different genens in circSCMH1-treated primary neurons by RNA-seq.
Contributor(s) Yang L, Yao H
Citation(s) 32441115
Submission date Jul 30, 2019
Last update date Jul 28, 2021
Contact name Li Yang
Organization name Southeast University
Department Pharmacology
Lab Pharmacology
Street address Dingjiaqiao 87
City Nanjing
State/province Jiangsu
ZIP/Postal code 21009
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM3986288 Vector-1
GSM3986289 Vector-2
GSM3986290 Vector-3
BioProject PRJNA557474
SRA SRP216852

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Supplementary file Size Download File type/resource
GSE135121_1_genes_fpkm_expression.xlsx 9.0 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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