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| Status |
Public on Aug 10, 2021 |
| Title |
Astrocyte alterations that accompany neuroinflammation induced anhedonia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The Khakh laboratory sequenced RNA from both whole and astrocyte specific RNAs from mouse prefrontal cortex and visual cortex by using Aldh1l1-CreERT2 - RiboTag mice. RNA was purified from controls and LPS injected mice, 1, 2 and 14 days after the injection.
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| Overall design |
Mice were injected i.p. with PBS or LPS (5 mg/kg). 1, 2 and 14 days after the injection PFC and VCX were homogenized and RNA was purified from (i) cleared lysate as the input, and (ii) astrocyte-specific ribosome-associated RNA precipitated via a hemagglutinin (HA) tag. LPS samples were compared to PBS samples. PFC samples were compared to VCX samples.
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| Contributor(s) |
Diaz-Castro B, Bernstein AM, Coppola G, Sofroniew MV, Khakh BS |
| Citation(s) |
34380036 |
| |
| Submission date |
Jul 30, 2019 |
| Last update date |
Sep 14, 2021 |
| Contact name |
Giovanni Coppola |
| E-mail(s) |
gcoppola@ucla.edu
|
| Phone |
310-794-4172
|
| Organization name |
UCLA
|
| Department |
Psychiatry and Neurology
|
| Lab |
Neurogenetics
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| Street address |
1524 Gonda, 695 Charles Young Drive South
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (102)
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| Relations |
| BioProject |
PRJNA557455 |
| SRA |
SRP216843 |
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