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Series GSE135108 Query DataSets for GSE135108
Status Public on Nov 05, 2019
Title Extracellular matrix hydrogel derived from decellularized tissues enables endoderm organoids culture
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Organoids have huge therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, the clinical application is greatly limited by the lack of effective GMP-complaint systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix hydrogels derived from decellularized tissues (DT) can provide the instructive native environment necessary to direct cell growth. These gels possess the biochemical signature of tissue-specific ECM, and have the potential for clinical translation due to their natural, non-carcinogenic origin. We show that gels developed from decellularized porcine small intestinal mucosa have similar physiological range and mechanical properties to commercially available gels. ECM-derived gels enable formation and growth of endoderm-derived organoids and, moreover, supports in vivo organoid growth. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.
Overall design RNA-seq profiles of organoids expanded on a decellularized porcine small intestinal mucosa-derived gel respect to control on Matrigel, with 4 replicates from the same pediatric small intestine cell line.
Contributor(s) Giobbe GG, Luni C, Crowley C, Campinoti S, Khedr M, De Santis M, Zambaiti E, Hu Q, Kretzschmar K, Urbani L, Meran L, Michielin F, Manfredi A, Cacchiarelli D, Eaton S, Li V, Clever H, Bonfanti P, Elvassore N, De Coppi P
Citation(s) 31827102
Submission date Jul 30, 2019
Last update date Feb 11, 2020
Contact name Lucio Di Filippo
Organization name TIGEM
Street address Via Campi Flegrei, 34
City Pozzuoli
State/province NA
ZIP/Postal code 80078
Country Italy
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (8)
GSM3986049 RNAseq_SI_MRF_r1
GSM3986050 RNAseq_SI_MRF_r2
GSM3986051 RNAseq_SI_MRF_r3
BioProject PRJNA557448
SRA SRP216842

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135108_RAW.tar 920.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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