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Series GSE135087 Query DataSets for GSE135087
Status Public on Jan 26, 2021
Title Bach2 deficiency leads to spontaneous expansion of IL-4-producing T follicular helper cells and autoimmunity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The transcription factor Bach2 is a critical negative regulator of Tfh cell differentiation, especially IL-4 subset. Tfh cells from the mesenteric lymph nodes of WT and Bach2 CD4 conditional KO mice were collected to process the Rna-seq. Mechanistically, Bach2 may limit abnormal IL-4-produicng Tfh cell formation by repressing c-Maf, CXCR5 and IL-4.
Overall design 10000-30000 Tfh Cells (CD4+B220-CD44+PD-1+CXCR5+) were sorted from the MLNs of WT and Bach2fl/flCd4-cre mice. cDNA was synthesized and amplified using the SMARTer Ultra Low RNA Kit for Illumina Sequencing (Clontech). The library for mRNA sequencing was generated using Illumnia TruSeq Preparation Kit (RS-122-2001) and sequenced using Illumnia Nextseq500 sequencer.
Contributor(s) Huang C, Zhang M, Zhang H
Citation(s) 31552021
Submission date Jul 30, 2019
Last update date Jan 26, 2021
Contact name Heng Zhang
Organization name Shanghai Jiao Tong University School of Medicine
Street address No.227 Sounth Chongqing Road
City Shanghai
ZIP/Postal code 200025
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM3985187 tfh_wt1: WT rep1
GSM3985188 tfh_wt2: WT rep2
GSM3985189 tfh_wt3: WT rep3
BioProject PRJNA557414
SRA SRP216829

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135087_tfh_normalized_readcount.csv.gz 537.0 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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