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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 24, 2019 |
| Title |
Stag1 and Stag2 regulate cell fate decisions in hematopoiesis through non-redundant topological control [II] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcriptional regulators, including the cohesin complex member STAG2, are recurrently mutated in cancer. The role of STAG2 in gene regulation, hematopoiesis, and tumor suppression remains unresolved. We show Stag2 deletion in hematopoietic stem/progenitor cells (HSPC) results in altered hematopoietic function, increased self-renewal, and impaired differentiation. ChIP-sequencing revealed that while Stag2 and Stag1 can bind the same loci, a component of Stag2 binding sites are unoccupied by Stag1 even in Stag2-deficient HSPCs. While concurrent loss of Stag2 and Stag1 abrogated hematopoiesis, Stag2 loss alone decreased chromatin accessibility and transcription of lineage-specification genes, including Ebf1 and Pax5, leading to blunted HSPC commitment to the B-cell lineage. Our data illustrate a role for Stag2 in transformation and transcriptional dysregulation distinct from its shared role with Stag1 in chromosomal segregation.
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| Overall design |
Single-cell RNA sequencing was performed on 6 samples ( Lin-HSPC) from Stag2 KO (n=3) and WT (n=3) mice and were assayed for transcriptome-wide RNA-sequence.
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| Contributor(s) |
Viny A, Lavallée V, Levine RL |
| Citation(s) |
31495782 |
| Submission date |
Jul 29, 2019 |
| Last update date |
Sep 25, 2019 |
| Contact name |
Richard Koche |
| E-mail(s) |
kocher@mskcc.org
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| Organization name |
Memorial Sloan Kettering Cancer Center
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| Street address |
417 E. 68th St.
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| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE135031 |
Stag1 and Stag2 regulate cell fate decisions in hematopoiesis through non-redundant topological control |
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| Relations |
| BioProject |
PRJNA557111 |
| SRA |
SRP216650 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE134997_RAW.tar |
62.4 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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