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Series GSE134997 Query DataSets for GSE134997
Status Public on Sep 24, 2019
Title Stag1 and Stag2 regulate cell fate decisions in hematopoiesis through non-redundant topological control [II]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Transcriptional regulators, including the cohesin complex member STAG2, are recurrently mutated in cancer. The role of STAG2 in gene regulation, hematopoiesis, and tumor suppression remains unresolved. We show Stag2 deletion in hematopoietic stem/progenitor cells (HSPC) results in altered hematopoietic function, increased self-renewal, and impaired differentiation. ChIP-sequencing revealed that while Stag2 and Stag1 can bind the same loci, a component of Stag2 binding sites are unoccupied by Stag1 even in Stag2-deficient HSPCs. While concurrent loss of Stag2 and Stag1 abrogated hematopoiesis, Stag2 loss alone decreased chromatin accessibility and transcription of lineage-specification genes, including Ebf1 and Pax5, leading to blunted HSPC commitment to the B-cell lineage. Our data illustrate a role for Stag2 in transformation and transcriptional dysregulation distinct from its shared role with Stag1 in chromosomal segregation.
Overall design Single-cell RNA sequencing was performed on 6 samples ( Lin-HSPC) from Stag2 KO (n=3) and WT (n=3) mice and were assayed for transcriptome-wide RNA-sequence.
Contributor(s) Viny A, Lavallée V, Levine RL
Citation(s) 31495782
Submission date Jul 29, 2019
Last update date Sep 25, 2019
Contact name Richard Koche
Organization name Memorial Sloan Kettering Cancer Center
Street address 417 E. 68th St.
City New York
State/province New York
ZIP/Postal code 10065
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM3983390 Lin- HSPC, Stag2 KO, XA1
GSM3983391 Lin- HSPC, Stag2 KO, XA2
GSM3983392 Lin- HSPC, Stag2 wt, XA3
This SubSeries is part of SuperSeries:
GSE135031 Stag1 and Stag2 regulate cell fate decisions in hematopoiesis through non-redundant topological control
BioProject PRJNA557111
SRA SRP216650

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Supplementary file Size Download File type/resource
GSE134997_RAW.tar 62.4 Mb (http)(custom) TAR (of CSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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