GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE134993 Query DataSets for GSE134993
Status Public on Nov 04, 2019
Title Genome-wide changes in OGT-deficient mouse liver tissue
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Over a billion people suffer from chronic liver diseases worldwide, which often leads to fibrosis and then cirrhosis. Treatments for fibrosis remain experimental, in part because no unifying mechanism has been identified that initiates liver fibrosis. O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) plays a pro-survival role under stress in many tissues. Here we report that OGT protects against hepatocyte necroptosis and initiation of liver fibrosis. Decreased O-GlcNAc levels were seen in patients with alcoholic liver cirrhosis and in mice with ethanol-induced liver injury. Liver-specific O-GlcNAc transferase (OGT) knockout (OGT-LKO) mice progressed to liver fibrosis at 10 weeks of age. OGT-deficient hepatocytes underwent necroptosis. These findings identify OGT as a key suppressor of hepatocyte necroptosis and OGT-LKO mice may serve as an effective spontaneous genetic model of liver fibrosis.
Overall design Total RNA was harvested from the liver tissue of 5-week-old OGT-deleted mice (n=4) and their control littermates (WT, n=5). Both genders were used for the study. Mice were fasted for 6 h before sacrifice.
Contributor(s) Zhang B, Nam JH, Chung D, Yang X
Citation(s) 31672932, 34146542
Submission date Jul 28, 2019
Last update date Jul 20, 2021
Contact name Xiaoyong Yang
Organization name Yale University
Department Comparative Medicine
Street address 375 Congress Ave
City New Haven
State/province CT
ZIP/Postal code 06520-8016
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (9)
GSM3983365 Wildtype liver tissue [OGT_WT_1]
GSM3983366 Wildtype liver tissue [OGT_WT_2]
GSM3983367 Wildtype liver tissue [OGT_WT_3]
BioProject PRJNA557230
SRA SRP216750

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134993_RAW.tar 920.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap