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Series GSE134937 Query DataSets for GSE134937
Status Public on Jun 30, 2020
Title Tandem CAR and single CAR T cells have different fates and therapeutic potency
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Chimeric antigen receptor T (CAR T) cells targeting CD19 have achieved breakthroughs in the treatment of haematological malignancies, but many clinical studies have also shown that a proportion of patients relapse after remission. In this study, we designed a series of tandem CARs (TanCARs) and found that TanCAR7 T cells retained relatively potent antitumour activity compared with single CAR T cell upon target antigen recognition. It may be associated with stable immunological synapse formation and rapid degranulation. Our transcriptional analysis underscores the potential of scFv domains binding to direct different T cell fates.
Overall design In order to assess the different phenotypic and functional patterns of CARs between TanCAR7 and single CAR, we compared the genome-wide transcriptional profiles of TanCAR7, CD19 CAR and CD20 CAR T cell after cocultured with Raji cells for 24 h.
Contributor(s) Chuan T
Citation(s) 32556247
Submission date Jul 26, 2019
Last update date Oct 14, 2020
Contact name Tong Chuan
Organization name Chinese PLA General Hospital
Department Molecular Biology and Immunology
Street address No. 28 Fuxing Road
City Beijing
State/province Beijing
ZIP/Postal code 100853
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM3979099 Tancar7_1
GSM3979100 Tancar7_2
GSM3979101 CAR19_1
BioProject PRJNA556849
SRA SRP216503

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134937_CRT.6sams.fpkm.txt.gz 413.7 Kb (ftp)(http) TXT
GSE134937_CRT.6sams.nodup_readcount.txt.gz 251.8 Kb (ftp)(http) TXT
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Processed data are available on Series record

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