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Series GSE134895 Query DataSets for GSE134895
Status Public on May 22, 2020
Title The microRNA miR-22 represses T helper 17 cell pathogenicity by targeting PTEN-regulated pathways
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Multiple sclerosis is a chronic autoimmune disease driven by pathogenic Th17 cells. Here, we dissected the role of miR-22 in pathogenic Th17 cells by autoantigen-specific disease models. We first showed that miR-22 was upregulated in peripheral lymphoid organs and spinal cords of mice developed autoimmune encephalomyelitis. Although miR-22 was upregulated in multiple helper T cell subsets, it was dispensable for T helper cell differentiation in vitro. While miR-22-/- mice exhibited milder symptoms of disease in an active EAE model, adoptive transfer of miR-22-/- 2D2 Th17 cells into naive recipient mice promoted higher disease incidence and severity compared to mice transferred with control 2D2 Th17 cells. Global transcriptional analysis of miR-22-deficient pathogenic Th17 cells revealed upregulated genes in phosphatase and tensin homologue (PTEN)-mediated pathways, and Pten was further identified as one of its potential targets. Therefore, we identified that Th17 cell intrinsic-miR-22 could protect mice from autoimmunity by targeting PTEN-regulated pathways.
Overall design RNA-seq for Mir-22 wild-type and knock-out in Th17 (23) cells with 3 replicates.
Contributor(s) Wang L, Qiu R, Zhang Z, Han Z, Yao C, Hou G, Dai D, Jin W, Tang Y, Yu X, Shen N
Citation(s) 32518131
Submission date Jul 25, 2019
Last update date Aug 21, 2020
Contact name Zhijun Han
Organization name Southern University of Science and Technology
Street address No 1088,xueyuan Rd., Nanshan District
City Shenzhen
State/province Guangdong
ZIP/Postal code 518055
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM3976570 Th17(23)_miR-22-/- RNA-seq rep1
GSM3976571 Th17(23)_miR-22-/- RNA-seq rep2
GSM3976572 Th17(23)_miR-22-/- RNA-seq rep3
BioProject PRJNA556698
SRA SRP216410

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GSE134895_RAW.tar 600.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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