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Series GSE134787 Query DataSets for GSE134787
Status Public on Jul 25, 2019
Title Differential expression levels of mRNAs in control 4T1 versus M2 macrophages co-cultured 4T1 murine breast cancer cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Tumor-associated macrophages (TAMs) are closely related to poor prognosis in triple-negative breast cancer (TNBC). Thus, gaining insight into how TAMs support cancer progression could contribute to effective therapies. We utilized the 4T1 murine TNBC cell line and murine bone marrow-derived macrophages to assess TAMs mediated pro-proliferative effects in vivo and in vitro. Further, Transcriptional analysis was performed to identify pathways activated in TAMs stimulated 4T1 cells. To simulate tumor microenvironment, M2 macrophages and 4T1 cells were plated into upper and lower chambers of Transwell co-culture systems respectively. we performed RNA-sequencing analysis of 4T1 cells incubated with vehicle control or M2 macrophages.
Overall design A high throughput sequencing about mRNAs was perfomed in control 4T1 versus M2 macrophages co-cultured 4T1 cells´╝î without replicate.
Contributor(s) Zhang Q, Zhou J, Le K, Xu M, Huang T
Citation(s) 31480004
Submission date Jul 24, 2019
Last update date Oct 27, 2019
Contact name zhang qiulei
Phone 13026173040
Organization name Huazhong university of science and technology
Department union hospital
Street address No. 1277, Jiefang Road,
City Wuhan
State/province hubei
ZIP/Postal code 430022
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM3968756 control_4T1
GSM3968757 M2_4T2
BioProject PRJNA556415
SRA SRP216227

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Supplementary file Size Download File type/resource
GSE134787_gene.tpm.matrix.txt.gz 234.9 Kb (ftp)(http) TXT
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