|
| Status |
Public on Jun 08, 2020 |
| Title |
The Evolving Immune Microenvironment of B-cell Acute Lymphoblastic Leukemia [murine dataset] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single-cell RNA-seq analysis of murine bone marrow and peripheral blood mononuclear cells (PBMCs) immune populations isolated from B-ALL recipient animals and two healthy littermate controls.
|
| |
|
| Overall design |
scRNA-Seq: For healthy recipients, processed bone marrow or peripheral blood cells were separated into GFP-CD45+Ly-6G- non-granulocytic cells and GFP-CD45+Ly-6G- non-granulocytic cells, then mixed at a ratio of 8 parts GFP-CD45+Ly-6G- non-granulocytic cells and 1 part GFP-CD45+Ly-6G+ granulocytic cells. For B-ALL recipients, cells were sorted and mixed to give 1 part GFP+CD19+ B-ALL blasts, 8 parts GFP-CD45+Ly-6G- non-granulocytic cells, and 1 part GFP-CD45+Ly-6G+ granulocytic cells.
|
| |
|
| Contributor(s) |
Witkowski MT, Dolgalev I, Tsirigos A, Aifantis I |
| Citation(s) |
32470390 |
| Submission date |
Jul 23, 2019 |
| Last update date |
Jun 08, 2020 |
| Contact name |
Igor Dolgalev |
| Organization name |
NYU Grossman School of Medicine
|
| Street address |
550 1st Ave
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (4)
|
|
| This SubSeries is part of SuperSeries: |
| GSE134759 |
The Evolving Immune Microenvironment of Human B-cell Acute Lymphoblastic Leukemia |
|
| Relations |
| BioProject |
PRJNA556280 |
| SRA |
SRP216169 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE134758_BALL-BM.dense_umis.tsv.gz |
101.3 Kb |
(ftp)(http) |
TSV |
| GSE134758_BALL-BM.matrix.mtx.gz |
167.2 Mb |
(ftp)(http) |
MTX |
| GSE134758_BALL-PB.dense_umis.tsv.gz |
90.1 Kb |
(ftp)(http) |
TSV |
| GSE134758_BALL-PB.matrix.mtx.gz |
143.5 Mb |
(ftp)(http) |
MTX |
| GSE134758_CON-BM.dense_umis.tsv.gz |
77.9 Kb |
(ftp)(http) |
TSV |
| GSE134758_CON-BM.matrix.mtx.gz |
133.3 Mb |
(ftp)(http) |
MTX |
| GSE134758_CON-PB.dense_umis.tsv.gz |
31.7 Kb |
(ftp)(http) |
TSV |
| GSE134758_CON-PB.matrix.mtx.gz |
50.2 Mb |
(ftp)(http) |
MTX |
| GSE134758_barcode_sample_metadata.txt.gz |
389 b |
(ftp)(http) |
TXT |
| GSE134758_barcodes.mm10.tsv.gz |
20.0 Mb |
(ftp)(http) |
TSV |
| GSE134758_features.mm10.tsv.gz |
272.8 Kb |
(ftp)(http) |
TSV |
| GSE134758_mm.counts.normalized.csv.gz |
204.7 Mb |
(ftp)(http) |
CSV |
| GSE134758_mm.counts.raw.csv.gz |
70.4 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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