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Series GSE134758 Query DataSets for GSE134758
Status Public on Jun 08, 2020
Title The Evolving Immune Microenvironment of B-cell Acute Lymphoblastic Leukemia [murine dataset]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Single-cell RNA-seq analysis of murine bone marrow and peripheral blood mononuclear cells (PBMCs) immune populations isolated from B-ALL recipient animals and two healthy littermate controls.
Overall design scRNA-Seq: For healthy recipients, processed bone marrow or peripheral blood cells were separated into GFP-CD45+Ly-6G- non-granulocytic cells and GFP-CD45+Ly-6G- non-granulocytic cells, then mixed at a ratio of 8 parts GFP-CD45+Ly-6G- non-granulocytic cells and 1 part GFP-CD45+Ly-6G+ granulocytic cells. For B-ALL recipients, cells were sorted and mixed to give 1 part GFP+CD19+ B-ALL blasts, 8 parts GFP-CD45+Ly-6G- non-granulocytic cells, and 1 part GFP-CD45+Ly-6G+ granulocytic cells.
Contributor(s) Witkowski MT, Dolgalev I, Tsirigos A, Aifantis I
Citation(s) 32470390
Submission date Jul 23, 2019
Last update date Jun 08, 2020
Contact name Igor Dolgalev
Organization name NYU Grossman School of Medicine
Street address 550 1st Ave
City New York
State/province New York
ZIP/Postal code 10016
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM3967069 CON1-BM, CON2-BM
This SubSeries is part of SuperSeries:
GSE134759 The Evolving Immune Microenvironment of Human B-cell Acute Lymphoblastic Leukemia
BioProject PRJNA556280
SRA SRP216169

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134758_BALL-BM.dense_umis.tsv.gz 101.3 Kb (ftp)(http) TSV
GSE134758_BALL-BM.matrix.mtx.gz 167.2 Mb (ftp)(http) MTX
GSE134758_BALL-PB.dense_umis.tsv.gz 90.1 Kb (ftp)(http) TSV
GSE134758_BALL-PB.matrix.mtx.gz 143.5 Mb (ftp)(http) MTX
GSE134758_CON-BM.dense_umis.tsv.gz 77.9 Kb (ftp)(http) TSV
GSE134758_CON-BM.matrix.mtx.gz 133.3 Mb (ftp)(http) MTX
GSE134758_CON-PB.dense_umis.tsv.gz 31.7 Kb (ftp)(http) TSV
GSE134758_CON-PB.matrix.mtx.gz 50.2 Mb (ftp)(http) MTX
GSE134758_barcode_sample_metadata.txt.gz 389 b (ftp)(http) TXT
GSE134758_barcodes.mm10.tsv.gz 20.0 Mb (ftp)(http) TSV
GSE134758_features.mm10.tsv.gz 272.8 Kb (ftp)(http) TSV
GSE134758_mm.counts.normalized.csv.gz 204.7 Mb (ftp)(http) CSV
GSE134758_mm.counts.raw.csv.gz 70.4 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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