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Series GSE134757 Query DataSets for GSE134757
Status Public on Jun 08, 2020
Title The Evolving Immune Microenvironment of B-cell Acute Lymphoblastic Leukemia [human peripheral blood dataset]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Single-cell RNA-seq analysis of primary human peripheral blood mononuclear cells (PBMCs) from two independent B-ALL patients (Texas Children's Hospital) and healthy PBMC donor samples (STEMCELL Technologies).
Overall design Single-cell RNA-sequencing libraries of primary human peripheral blood immune populations. B-ALL patient bone marrow samples were separated into CD19+ B cell and CD19-CD45+ non-B cell fractions by flow-based sorting and then mixed at a ratio of 1:5 CD19+:CD19-CD45+ followed by scRNA-Seq. Total CD45+ peripheral blood cells were sequenced from healthy donors.
Contributor(s) Witkowski MT, Dolgalev I, Tsirigos A, Aifantis I
Citation(s) 32470390
Submission date Jul 23, 2019
Last update date Jun 08, 2020
Contact name Igor Dolgalev
Organization name NYU Grossman School of Medicine
Street address 550 1st Ave
City New York
State/province New York
ZIP/Postal code 10016
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (1)
This SubSeries is part of SuperSeries:
GSE134759 The Evolving Immune Microenvironment of Human B-cell Acute Lymphoblastic Leukemia
BioProject PRJNA556277
SRA SRP216168

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Supplementary file Size Download File type/resource
GSE134757_barcode_sample_metadata.txt.gz 431 b (ftp)(http) TXT
GSE134757_barcodes.hg38.tsv.gz 20.0 Mb (ftp)(http) TSV
GSE134757_features.hg38.tsv.gz 297.6 Kb (ftp)(http) TSV
GSE134757_hPB.counts.normalized.csv.gz 47.9 Mb (ftp)(http) CSV
GSE134757_hPB.counts.raw.csv.gz 16.8 Mb (ftp)(http) CSV
GSE134757_hPB.dense_umis.tsv.gz 147.1 Kb (ftp)(http) TSV
GSE134757_hPB.matrix.mtx.gz 118.7 Mb (ftp)(http) MTX
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Processed data are available on Series record

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