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| Status |
Public on Jun 03, 2020 |
| Title |
In vitro transcriptomic responses to thioacetamide-S-oxide exposure in human primary hepatocytes, renal tube epithelial, and cardiomyocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study we tested the ability to predict organ injury endpoints from in vitro transcriptomics responses at early time points (9 and 24 hours) after to thioacetamide-S-oxide treatment, the toxic metabolite of thioacetamide. Thioacetamide, an organosulfur compound, have been extensively used in animal studies as a hepatotoxin and carcinogen for its ability to cause acute liver damage.
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| Overall design |
We treated three human cell types, primary hepatocytes (vehicle, low (0.50 mM), or high (0.25 mM) dose), renal tube epithelial cells (vehicle, low (0.25 mM), or high (1.00 mM) dose), and cardiomyocytes (vehicle, low (0.25 mM), or high (0.75 mM) dose) with thioacetamide-S-oxide. RNA-seq data were collected 9 and 24 hours after application of vehicle or thioacetamide-S-oxide.
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| Contributor(s) |
Schyman P, Printz RL, Estes SK, O’Brien TP, Shiota M, Wallqvist A |
| Citation(s) |
32512829 |
| Submission date |
Jul 22, 2019 |
| Last update date |
Jun 29, 2020 |
| Contact name |
Patric Schyman |
| E-mail(s) |
patric.schyman@gmail.com
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| Phone |
3016191978
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| Organization name |
Biotechnology HPC Software Applications Institute (BHSAI)
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| Street address |
2405 Whittier Drive, Suite 200
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| City |
Frederick |
| State/province |
Maryland |
| ZIP/Postal code |
21702 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (90)
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| GSM3960735 |
Primary hepatocytes, Vehicle, 9 h (ms-1) |
| GSM3960736 |
Primary hepatocytes, Vehicle, 9 h (ms-2) |
| GSM3960737 |
Primary hepatocytes, Vehicle, 9 h (ms-3) |
| GSM3960738 |
Primary hepatocytes, Vehicle, 9 h (ms-4) |
| GSM3960739 |
Primary hepatocytes, Vehicle, 9 h (ms-5) |
| GSM3960740 |
Primary hepatocytes, Treated, Low, 9 h (ms-6) |
| GSM3960741 |
Primary hepatocytes, Treated, Low, 9 h (ms-7) |
| GSM3960742 |
Primary hepatocytes, Treated, Low, 9 h (ms-8) |
| GSM3960743 |
Primary hepatocytes, Treated, Low, 9 h (ms-9) |
| GSM3960744 |
Primary hepatocytes, Treated, Low, 9 h (ms-10) |
| GSM3960745 |
Primary hepatocytes, Treated, High, 9 h (ms-11) |
| GSM3960746 |
Primary hepatocytes, Treated, High, 9 h (ms-12) |
| GSM3960747 |
Primary hepatocytes, Treated, High, 9 h (ms-13) |
| GSM3960748 |
Primary hepatocytes, Treated, High, 9 h (ms-14) |
| GSM3960749 |
Primary hepatocytes, Treated, High, 9 h (ms-15) |
| GSM3960750 |
Primary hepatocytes, Vehicle, 24 h (ms-16) |
| GSM3960751 |
Primary hepatocytes, Vehicle, 24 h (ms-17) |
| GSM3960752 |
Primary hepatocytes, Vehicle, 24 h (ms-18) |
| GSM3960753 |
Primary hepatocytes, Vehicle, 24 h (ms-19) |
| GSM3960754 |
Primary hepatocytes, Vehicle, 24 h (ms-20) |
| GSM3960755 |
Primary hepatocytes, Treated, Low, 24 h (ms-21) |
| GSM3960756 |
Primary hepatocytes, Treated, Low, 24 h (ms-22) |
| GSM3960757 |
Primary hepatocytes, Treated, Low, 24 h (ms-23) |
| GSM3960758 |
Primary hepatocytes, Treated, Low, 24 h (ms-24) |
| GSM3960759 |
Primary hepatocytes, Treated, Low, 24 h (ms-25) |
| GSM3960760 |
Primary hepatocytes, Treated, High, 24 h (ms-26) |
| GSM3960761 |
Primary hepatocytes, Treated, High, 24 h (ms-27) |
| GSM3960762 |
Primary hepatocytes, Treated, High, 24 h (ms-28) |
| GSM3960763 |
Primary hepatocytes, Treated, High, 24 h (ms-29) |
| GSM3960764 |
Primary hepatocytes, Treated, High, 24 h (ms-30) |
| GSM3960765 |
Renal tube epithelial cells, Vehicle, 9 h (ms-31) |
| GSM3960766 |
Renal tube epithelial cells, Vehicle, 9 h (ms-32) |
| GSM3960767 |
Renal tube epithelial cells, Vehicle, 9 h (ms-33) |
| GSM3960768 |
Renal tube epithelial cells, Vehicle, 9 h (ms-34) |
| GSM3960769 |
Renal tube epithelial cells, Vehicle, 9 h (ms-35) |
| GSM3960770 |
Renal tube epithelial cells, Treated, Low, 9 h (ms-36) |
| GSM3960771 |
Renal tube epithelial cells, Treated, Low, 9 h (ms-37) |
| GSM3960772 |
Renal tube epithelial cells, Treated, Low, 9 h (ms-38) |
| GSM3960773 |
Renal tube epithelial cells, Treated, Low, 9 h (ms-39) |
| GSM3960774 |
Renal tube epithelial cells, Treated, Low, 9 h (ms-40) |
| GSM3960775 |
Renal tube epithelial cells, Treated, High, 9 h (ms-41) |
| GSM3960776 |
Renal tube epithelial cells, Treated, High, 9 h (ms-42) |
| GSM3960777 |
Renal tube epithelial cells, Treated, High, 9 h (ms-43) |
| GSM3960778 |
Renal tube epithelial cells, Treated, High, 9 h (ms-44) |
| GSM3960779 |
Renal tube epithelial cells, Treated, High, 9 h (ms-45) |
| GSM3960780 |
Renal tube epithelial cells, Vehicle, 24 h (ms-46) |
| GSM3960781 |
Renal tube epithelial cells, Vehicle, 24 h (ms-47) |
| GSM3960782 |
Renal tube epithelial cells, Vehicle, 24 h (ms-48) |
| GSM3960783 |
Renal tube epithelial cells, Vehicle, 24 h (ms-49) |
| GSM3960784 |
Renal tube epithelial cells, Vehicle, 24 h (ms-50) |
| GSM3960785 |
Renal tube epithelial cells, Treated, Low, 24 h (ms-51) |
| GSM3960786 |
Renal tube epithelial cells, Treated, Low, 24 h (ms-52) |
| GSM3960787 |
Renal tube epithelial cells, Treated, Low, 24 h (ms-53) |
| GSM3960788 |
Renal tube epithelial cells, Treated, Low, 24 h (ms-54) |
| GSM3960789 |
Renal tube epithelial cells, Treated, Low, 24 h (ms-55) |
| GSM3960790 |
Renal tube epithelial cells, Treated, High, 24 h (ms-56) |
| GSM3960791 |
Renal tube epithelial cells, Treated, High, 24 h (ms-57) |
| GSM3960792 |
Renal tube epithelial cells, Treated, High, 24 h (ms-58) |
| GSM3960793 |
Renal tube epithelial cells, Treated, High, 24 h (ms-59) |
| GSM3960794 |
Renal tube epithelial cells, Treated, High, 24 h (ms-60) |
| GSM3960795 |
Cardiomyocytes, Vehicle, 9 h (ms-61) |
| GSM3960796 |
Cardiomyocytes, Vehicle, 9 h (ms-62) |
| GSM3960797 |
Cardiomyocytes, Vehicle, 9 h (ms-63) |
| GSM3960798 |
Cardiomyocytes, Vehicle, 9 h (ms-64) |
| GSM3960799 |
Cardiomyocytes, Vehicle, 9 h (ms-65) |
| GSM3960800 |
Cardiomyocytes, Treated, Low, 9 h (ms-66) |
| GSM3960801 |
Cardiomyocytes, Treated, Low, 9 h (ms-67) |
| GSM3960802 |
Cardiomyocytes, Treated, Low, 9 h (ms-68) |
| GSM3960803 |
Cardiomyocytes, Treated, Low, 9 h (ms-69) |
| GSM3960804 |
Cardiomyocytes, Treated, Low, 9 h (ms-70) |
| GSM3960805 |
Cardiomyocytes, Treated, High, 9 h (ms-71) |
| GSM3960806 |
Cardiomyocytes, Treated, High, 9 h (ms-72) |
| GSM3960807 |
Cardiomyocytes, Treated, High, 9 h (ms-73) |
| GSM3960808 |
Cardiomyocytes, Treated, High, 9 h (ms-74) |
| GSM3960809 |
Cardiomyocytes, Treated, High, 9 h (ms-75) |
| GSM3960810 |
Cardiomyocytes, Vehicle, 24 h (ms-76) |
| GSM3960811 |
Cardiomyocytes, Vehicle, 24 h (ms-77) |
| GSM3960812 |
Cardiomyocytes, Vehicle, 24 h (ms-78) |
| GSM3960813 |
Cardiomyocytes, Vehicle, 24 h (ms-79) |
| GSM3960814 |
Cardiomyocytes, Vehicle, 24 h (ms-80) |
| GSM3960815 |
Cardiomyocytes, Treated, Low, 24 h (ms-81) |
| GSM3960816 |
Cardiomyocytes, Treated, Low, 24 h (ms-82) |
| GSM3960817 |
Cardiomyocytes, Treated, Low, 24 h (ms-83) |
| GSM3960818 |
Cardiomyocytes, Treated, Low, 24 h (ms-84) |
| GSM3960819 |
Cardiomyocytes, Treated, Low, 24 h (ms-85) |
| GSM3960820 |
Cardiomyocytes, Treated, High, 24 h (ms-86) |
| GSM3960821 |
Cardiomyocytes, Treated, High, 24 h (ms-87) |
| GSM3960822 |
Cardiomyocytes, Treated, High, 24 h (ms-88) |
| GSM3960823 |
Cardiomyocytes, Treated, High, 24 h (ms-89) |
| GSM3960824 |
Cardiomyocytes, Treated, High, 24 h (ms-90) |
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| Relations |
| BioProject |
PRJNA555991 |
| SRA |
SRP215908 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE134641_RAW.tar |
197.8 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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