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Series GSE134641 Query DataSets for GSE134641
Status Public on Jun 03, 2020
Title In vitro transcriptomic responses to thioacetamide-S-oxide exposure in human primary hepatocytes, renal tube epithelial, and cardiomyocytes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary In this study we tested the ability to predict organ injury endpoints from in vitro transcriptomics responses at early time points (9 and 24 hours) after to thioacetamide-S-oxide treatment, the toxic metabolite of thioacetamide. Thioacetamide, an organosulfur compound, have been extensively used in animal studies as a hepatotoxin and carcinogen for its ability to cause acute liver damage.
Overall design We treated three human cell types, primary hepatocytes (vehicle, low (0.50 mM), or high (0.25 mM) dose), renal tube epithelial cells (vehicle, low (0.25 mM), or high (1.00 mM) dose), and cardiomyocytes (vehicle, low (0.25 mM), or high (0.75 mM) dose) with thioacetamide-S-oxide. RNA-seq data were collected 9 and 24 hours after application of vehicle or thioacetamide-S-oxide.
Contributor(s) Schyman P, Printz RL, Estes SK, O’Brien TP, Shiota M, Wallqvist A
Citation(s) 32512829
Submission date Jul 22, 2019
Last update date Jun 29, 2020
Contact name Patric Schyman
Phone 3016191978
Organization name Biotechnology HPC Software Applications Institute (BHSAI)
Street address 2405 Whittier Drive, Suite 200
City Frederick
State/province Maryland
ZIP/Postal code 21702
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (90)
GSM3960735 Primary hepatocytes, Vehicle, 9 h (ms-1)
GSM3960736 Primary hepatocytes, Vehicle, 9 h (ms-2)
GSM3960737 Primary hepatocytes, Vehicle, 9 h (ms-3)
BioProject PRJNA555991
SRA SRP215908

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134641_RAW.tar 197.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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