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Series GSE134577 Query DataSets for GSE134577
Status Public on Nov 04, 2019
Title Clonally expanded CD8 T cells patrol Alzheimer's cerebrospinal fluid [CSF]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Alzheimer’s disease (AD) is an age-related neurodegenerative disorder in which neuroinflammation plays a critical function. Recurring viral infections and loss of immune competence increase risk for developing AD, yet the cellular and molecular mechanisms driving these immune changes are unknown. Here we performed mass cytometry of peripheral blood mononuclear cells and detected an immunologic signature of AD characterized by increased numbers of CD8+ T effector memory CD45RA+ (TEMRA) cells. CD8+ TEMRA cells were negatively associated with cognition and single cell RNA sequencing revealed their cytotoxic effector function. Strikingly, we discovered identical, shared T cell receptors (TCRs) of clonally expanded CD8+ TEMRA cells in cerebrospinal fluid (CSF) of three AD patients. Deep TCR sequencing, machine learning, and peptide screens identified the HLA-B*08:01-restricted Epstein-Barr virus trans-activator protein BZLF1 as the cognate antigen of a novel AD CSF TCR . These results provide the first evidence of clonal, antigen-specific T  cells patrolling the intrathecal space of brains affected by age-related neurodegeneration  
Overall design Examination of transcriptional profile of cells from the cerbrospinal fluid of healthy and diseased patients.

AD = Alzheimer's disease
MCI = mild cognitive impairment
HC = healthy control
Contributor(s) Gate D, Leventhal O
Citation(s) 31915375, 33482851
Submission date Jul 19, 2019
Last update date Feb 02, 2021
Contact name Olivia Leventhal
Organization name Stanford University
Department Neurology
Lab Wyss-Coray
Street address 1201 Welch Road, rm p220
City Stanford
State/province California (CA)
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (18)
GSM3984199 CSF_HC9
GSM3984200 CSF_AD6
GSM3984201 CSF_HC10
This SubSeries is part of SuperSeries:
GSE134578 Clonally expanded CD8 T cells patrol Alzheimer's cerebrospinal fluid
BioProject PRJNA557207
SRA SRP216709

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Supplementary file Size Download File type/resource
GSE134577_RAW.tar 85.1 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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