GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE134540 Query DataSets for GSE134540
Status Public on Sep 05, 2019
Title Muscle-secreted G-CSF as a metabolic niche factor ameliorates loss of muscle stem cell in aged mice (scRNA-seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Function and number of muscle stem cells (satellite cells, SCs) declines with muscle aging. Although SCs are heterogeneous and different subpopulations have been identified, it remains unknown if a specific subpopulation of muscle SCs selectively decreases during aging. Here, we find Pax7Hi cells are dramatically reduced in aged mice and this aged-dependent loss of Pax7Hi cells is metabolically mediated by myofiber-secreted granulocyte-colony stimulating factor G-CSF as the Pax7Hi SCs are replenished by exercise-induced G-CSF in aged mice. Mechanistically, we show that transcription of G-CSF (Csf3) gene in myofibers is regulated by MyoD in a metabolism-dependent manner and the myofibers-secreted G-CSF acts as a metabolic niche factor required for establishing and maintaining the Pax7Hi SC subpopulation in adult and physiological aged mice by promoting the asymmetric division of Pax7Hi and Pax7Mi SCs. Together, our findings uncover a metabolic niche role of muscle metabolism in regulating Pax7 SC heterogeneity in mice.
Overall design Three subpopulations of satellite cells (Pax7Hi, Pax7Mi and Pax7Lo) were isolated by FACS based on levels of GFP (Pax7) from tibialis anterior muscle of Pax7-nGFP transgenic mice and subjected to single cell RNA sequencing (scRNA-Seq).
Contributor(s) Li H, Chen Q, Li C, Zhong R, Zhao Y, Zhang Q, Tong W, Zhu D, Zhang Y
Citation(s) 31736098
Submission date Jul 19, 2019
Last update date Dec 10, 2019
Contact name Yong Zhang
Organization name School of Basic Medicine, Peking Union Medical College
Street address 5 Dong Dan San Tiao
City Beijing
ZIP/Postal code 100005
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (3)
GSM3955372 Pax7Hi
GSM3955373 Pax7Mi
GSM3955374 Pax7Lo
This SubSeries is part of SuperSeries:
GSE134253 Muscle-secreted G-CSF as a metabolic niche factor ameliorates loss of muscle stem cell in aged mice
BioProject PRJNA555526
SRA SRP215427

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134540_RAW.tar 16.3 Mb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap