GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE134403 Query DataSets for GSE134403
Status Public on Mar 01, 2020
Title Construction of Human Primary Epidermal Organoids and Modeling of Dermatophyte Infection
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Despite the strong need for the large-scale generation of human epidermal cells for cell therapy of extensive and severe skin injury, tissue-engineered skin construction and dermatological investigation, the effectiveness of current culture methods is limited and have yet to meet the huge demands sufficiently. Here, we report the establishment of a simple and robust 3D culture system using chemical defined, serum-free medium supplemented with small molecules and growth factors, tailored for human epidermal organoids generation and expansion. The conditions allowed 10,000-fold expansion of the primary epidermal cells within 6 weeks, with the cell architecture and gene expression preserved. We further noticed that only Integrin α6-positive basal stem cells freshly isolated from human epidermis could form expandable epidermal organoids. The epidermal cells inside of the organoids also have the potential to reconstruct multi-layered human epidermis equivalents under classical air-liquid interface condition. Remarkably, the epidermal organoids enable to model the anthropophilic and epidermis-restricted Trichophyton Rubrum infection. This model faithfully mimics and reflects the clinical pathological reactions of reinforced physical barrier by promoting keratinocytes differentiation and accelerated formation of the stratum corneum for adaptation and resistance to infection. Furthermore, the infection model also suggested that constraining host IL-1 signaling may underline the high degree of adaptation of Trichophyton Rubrum to human skin, and the tendency to be chronic and recurrent infection. Thus, human epidermal organoids present a powerful platform for modeling human dermatology, skin tissue engineering and cell therapy.
Overall design Evaluation of whole transcriptome of cells in 2 groups, each of them include 2 replication
Contributor(s) Wang Y, Wang S
Citation(s) 33414472
Submission date Jul 17, 2019
Last update date Jan 19, 2021
Contact name wang chuan wen
Organization name AnnoGene
Street address No. 288, Yang Cun road, Chou Jiang Street, Yiwu, Zhejiang
City YiWu
ZIP/Postal code 322000
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM3946043 Control-1
GSM3946044 Control-2
GSM3946045 T-rubrum-1
BioProject PRJNA555187
SRA SRP215190

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134403_fpkm.txt.gz 716.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap