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Series GSE134063 Query DataSets for GSE134063
Status Public on Nov 11, 2021
Title Regulation of gene expression by Ankrd35 in LKR10 cells grown in 2D monoculture or as 3D clusters in methylcellulose
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The objective of this study was to use RNAseq to determine which gene change in expression when LKR10 cells are grown in monolayers (2D) or methylcellulose on ultra low attachment plates (3D), which prevents attachment of cells to the plate and promotes formation of 3D sphere-like clusters. A secondary objective was to determine whether knockdown of Ankrd35 altered gene expression of specific genes in 2D or 3D.
Overall design Examination of gene expression in the murine LKR10 cell line with and without knockdown of Ankrd35 in traditional 2D monolayer cultures and in 3D methylcellulose cultures by RNAseq
Contributor(s) Simpson DR, Lee AG, Sweet-Cordero A
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Submission date Jul 10, 2019
Last update date Nov 11, 2021
Contact name David Simpson
Organization name UCSF
Street address 1550 4th Street
City San Francisco
State/province California
ZIP/Postal code 94158
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (16)
GSM3935839 LK2DG1: LKR10_2D_shGFP_Rep1
GSM3935840 LK2DG2: LKR10_2D_shGFP_Rep2
GSM3935841 LK2DG3: LKR10_2D_shGFP_Rep3
This SubSeries is part of SuperSeries:
GSE136346 Identification of Ankrd35 as a mediator of cisplatin response in non-small cell lung cancer by ex vivo 3D screening of primary tumor spheroids
BioProject PRJNA553670
SRA SRP214047

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Supplementary file Size Download File type/resource
GSE134063_Ankrd35KD2D3DGeneExpressionMatrix.tsv.gz 1.5 Mb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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