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Series GSE134061 Query DataSets for GSE134061
Status Public on Jul 11, 2019
Title MicroRNA profiling during neural differentiation of induced pluripotent stem cells
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary MicroRNAs (miRNA) play an essential role in the regulation of gene expression, influence signaling networks responsible for several cellular processes like differentiation of pluripotent stem cells. Despite several studies on the neurogenesis process, no global analysis of microRNA expression during differentiation of induced pluripotent stem cells (iPSC) to neuronal stem cells (NSC) has been done. Therefore we compared the profile of microRNA expression in iPSC lines and in NSC lines derived from them, using microarray-based analysis. Two different protocols for NSC formation were used: direct and two-step via neural rosette formation. We confirmed the new associations of previously described miRNAs in regulation of NSC differentiation from iPSC. We discovered upregulation of miR-10 family, miR-30 family and miR-9 family and downregulation of miR-302 and miR-515 family. Moreover we showed that miR-10 family play a crucial role in the negative regulation of genes expression belonging to signaling pathways involved in neural differentiation: WNT signaling pathway, focal adhesion, signaling pathways regulating pluripotency of stem cells.
Overall design We conducted differentiation of human induced pluripotent stem cells into neural stem cells. Then we carried out global miRNA expression analysis using the Affymetrix platform. We profiled the miRNAs which are crucial regulators of neural differentiation. We not only confirmed the involvement of previously described miRNAs characteristic for transition from ES to NSC: mir-302 family, hsa-miR-125b, hsa-miR-9, let-7, but we also pointed out others, which may be characteristic for iPSC to NSC transition.
Contributor(s) Kulcenty K, Wroblewska JP, Rucinski M, Kozlowska E, Jopek K, Suchorska WM
Citation(s) 31357387
Submission date Jul 10, 2019
Last update date Sep 11, 2019
Contact name Marcin Rucinski
Organization name Poznan University of Medical Science
Department Histology and Embriology
Street address Swiecicki 6
City Poznan
ZIP/Postal code 60-781
Country Poland
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (8)
GSM3935825 undifferentiated GPCCi001-A cell line, biol. rep 1
GSM3935826 undifferentiated ND41658*H cell line, biol. rep 1
GSM3935827 undifferentiated GPCCi001-A cell line, biol. rep 2
BioProject PRJNA553662

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Supplementary file Size Download File type/resource
GSE134061_RAW.tar 6.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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