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| Status |
Public on May 01, 2020 |
| Title |
Genome-wide maps of chromatin state in A375 cells or in normal or USP7 knockdown A375 cells . |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ChIP-seq experiments were performed in normal or USP7 knockdown A375 cells with specific antibodies against USP7, EZH2, H3K27me3 and H2BK120ub1. Consistent with our expectations, the analysis of ChIP-seq data showed that USP7 loss-of-function led to obviously reduction in USP7, EZH2 and H3K27me3 around the USP7-specific peaks, but an upregulation in H2BK120ub1. This study gave us a new understanding that USP7 is required for EZH2 loading and H3K27 trimethylation.
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| |
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| Overall design |
Examination of the change of the indicated protein densities around the USP7 specific peaks after knockdown of USP7
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| |
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| Contributor(s) |
Su D, Shan L |
| Citation(s) |
33849069 |
| |
| Submission date |
Jul 04, 2019 |
| Last update date |
May 20, 2021 |
| Contact name |
Lin Shan |
| E-mail(s) |
shanlin@ccmu.edu.cn
|
| Phone |
86-10-83950219
|
| Organization name |
Capital Medical University
|
| Street address |
10 You’anmen Outer West 1st Street
|
| City |
Beijing |
| ZIP/Postal code |
100094 |
| Country |
China |
| |
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| Platforms (2) |
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| Samples (23)
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| Relations |
| BioProject |
PRJNA552988 |
| SRA |
SRP213355 |
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