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Series GSE133756

Query DataSets for GSE133756

Status

Public on Jul 04, 2019

Title

Targeted RNA-sequencing Facilitates Quantitative Analysis of RB and E2F3 in mouse Sertoli cells

Organism

Experiment type

Expression profiling by high throughput sequencing

Summary

A Sertoli cell specific knockout of retinoblastoma protein (SC-RbKO) results in a complete disruption of spermatogenesis in mice. In this project the SC-RbKO mice were cross-bred with E2F3 floxed mice to see if a double knockout would rescue the phenotype associated with RB loss.

Overall design

Gene expression analysis by Targeted RNA-sequencing between SC-E2F3KO and Wild type at 2 and 5 months old testes and among SC-Rb-/-E2F3+/+, SC-Rb-/-E2F3+/-, SC-Rb-/-E2F3-/- and Wild type at 2,5 months old testes were performed.

Contributor(s)

Citation(s)

Submission date

Jul 03, 2019

Last update date

Oct 04, 2019

Contact name

Emmi Rotgers

E-mail(s)

Organization name

University of Turku

Department

Institute of Biomedicine

Lab

Jorma Toopari´s lab

Street address

Kiinamyllynkatu 10

City

Turku

ZIP/Postal code

20520

Country

Finland

Platforms (1)

Illumina MiSeq (Mus musculus)

Samples (44)

More...

GSM3926664	Testis, b.24.8 6.
GSM3926665	Testis, b. 6.10 3.
GSM3926666	Testis, b. 6.10. 2

GSM3926667	Testis, b. 8.9. 3.
GSM3926668	Testis, b. 24.8. 5.
GSM3926669	Testis, 3/6087 cre+ lox/lox
GSM3926670	Testis, 13/6092 cre+ lox/lox
GSM3926671	Testis, 3/6156 cre+ lox/lox
GSM3926672	Testis, 4/6336 cre+ lox/lox
GSM3926673	Testis, 1/6386 cre+ lox/lox
GSM3926674	Testis, 10/6264 cre+ lox/lox
GSM3926675	Testis, 3/6328 cre+ lox/lox
GSM3926676	Testis, 10/6329 cre+ lox/lox
GSM3926677	Testis, 10/6334 cre+ lox/lox
GSM3926678	Testis, 11/6458 cre + lox/lox
GSM3926679	Testis, 10/6088 cre- lox/lox
GSM3926680	Testis, 11/6091 cre- lox/lox
GSM3926681	Testis, 31/6093 cre- lox/lox
GSM3926682	Testis, 3/6147 cre-lox/wt
GSM3926683	Testis, 10/6420 cre- lox/wt
GSM3926684	Testis, 1/6155 cre- lox/lox
GSM3926685	Testis, 10/6256 cre- lox/lox
GSM3926686	Testis, 3/6343 cre- lox/wt
GSM3926687	Testis, 1/6350 cre- lox/wt
GSM3926688	Testis, 30/6335 cre- lox/lox
GSM3926689	Testis, 1/6777 cre+ E2F3 wt/wt Rb lox/lox
GSM3926690	Testis, 30/6780 cre+ E2F3 wt/wt Rb lox/lox
GSM3926691	Testis, 30/6849 cre+ E2F3 wt/wt Rb lox/lox
GSM3926692	Testis, 30/7007 cre+ E2F3 wt/wt Rb lox/lox
GSM3926693	Testis, 1/6612 cre+ E2F3 lox/wt Rb lox/lox
GSM3926694	Testis, 4/6679 cre+ E2F3 lox/wt Rb lox/lox
GSM3926695	Testis, 1/6729 cre+ E2F3 lox/wt Rb lox/lox
GSM3926696	Testis, 3/6730 cre+ E2F3 lox/wt Rb lox/lox
GSM3926697	Testis, 1/6797 cre+ E2F3 lox/wt Rb lox/lox
GSM3926698	Testis, 10/6930 cre+ E2F3 lox/lox Rb lox/lox
GSM3926699	Testis, 30/6931 cre+ E2F3 lox/lox Rb lox/lox
GSM3926700	Testis, 3/6969 cre+ E2F3 lox/lox Rb lox/lox
GSM3926701	Testis, 10/6977 cre+ E2F3 lox/lox Rb lox/lox
GSM3926702	Testis, 10/7037 cre+ E2F3 lox/lox Rb lox/lox
GSM3926703	Testis, 3/6613 cre- E2F3 lox/wt Rb lox/wt
GSM3926704	Testis, 30/6678 cre- E2F3 wt/wt Rb lox/lox
GSM3926705	Testis, 10/6731 cre- E2F3 lox/wt Rb lox/lox
GSM3926706	Testis, 30/6732 cre- E2F3 lox/wt Rb lox/lox
GSM3926707	Testis, 1/6738 E2F3 lox/lox Rb lox/lox

Relations

BioProject

SRA

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE133756_2.5_months_SC_RB_E2F3_NormalizedHitsPerSample_M1.tsv.gz	20.0 Kb	(ftp)(http)	TSV
GSE133756_2.5_months_SC_RB_E2F3_TargetHitsPerSample_M1.json.gz	6.8 Kb	(ftp)(http)	JSON
GSE133756_2_months_SC_E2F3_NormalizedHitsPerSample_M1.tsv.gz	9.5 Kb	(ftp)(http)	TSV
GSE133756_2_months_SC_E2F3_TargetHitsPerSample_M1.json.gz	4.1 Kb	(ftp)(http)	JSON
GSE133756_5_months_SC_E2F3_NormalizedHitsPerSample_M1.tsv.gz	9.6 Kb	(ftp)(http)	TSV
GSE133756_5_months_SC_E2F3_TargetHitsPerSample_M1.json.gz	4.1 Kb	(ftp)(http)	JSON
SRA Run Selector
Raw data are available in SRA
Processed data are available on Series record

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