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Series GSE133751 Query DataSets for GSE133751
Status Public on Apr 06, 2020
Title Comprehensive analyses of function and molecular interaction of differentially expressed non-coding RNAs and mRNA in Hantaan virus infection
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Hantaan virus (HTNV), the prevalent prototype of the hantavirus in Asia, causes hemorrhagic fever with renal syndrome (HFRS) with high mortality in human being. However, the pathogenesis of HTNV infection remains elusive. Accumulating evidences indicate that non-coding RNAs (ncRNAs), including long non-coding RNA (lncRNA), circular RNA (circRNA) and microRNA (miRNA) play crucial roles in the progression of virus infection. Here, we identified differential lncRNA/miRNA/circRNA and mRNA expression profiles of HTNV-infected  human umbilical vein endothelial cells (HUVECs) compared with mock-infected HUVECs by whole transcriptome sequencing. Subsequently, comprehensive bioinformatics analyses established miRNA-mRNA co-expression, protein-protein interaction and competing endogenous RNA (ceRNA) networks in miRNA-lncRNA-circRNA-mRNA regulatory axis. The trans or cis regulatory roles of identified RNAs on HTNV infection were ascertained by RNA interference and key ceRNA relationships were verified by dual-luciferase reporter experiments. Moreover, gene ontology (GO) enrichment analysis showed that dysregulated RNAs were mostly related to antiviral innate immune response. In conclusion, our findings firstly revealed that circRNAs and ceRNA network were involved in regulating HTNV infection, and also confirmed several key lncRNAs and miRNAs which had vital effects on HTNV infection. The identification and characterization of RNAs provide the new insights on ceRNA networks in HTNV-host interactions, which lays the foundation for future research of the potential roles of ncRNAs in the pathogenesis of HFRS.
Overall design Mock-infected and Hantaan virus-infected HUVECs were generated by deep sequencing, in triplicate respectively
Contributor(s) Lu S, Luo F, Hou W
Citation(s) 32232013
Submission date Jul 03, 2019
Last update date Apr 06, 2020
Contact name LI LI
Organization name wuhan university school of basic medical sciences
Street address 185 Donghu Road
City Wuhan
ZIP/Postal code 430071
Country China
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (6)
GSM3926641 HUVECs, con-3
GSM3926642 HUVECs, con-4
GSM3926643 HUVECs, con-5
BioProject PRJNA552458
SRA SRP212863

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Supplementary file Size Download File type/resource
GSE133751_lncRNA-exp.txt.gz 1.1 Mb (ftp)(http) TXT
GSE133751_mRNA-exp.txt.gz 2.0 Mb (ftp)(http) TXT
GSE133751_new_lncRNA-exp.txt.gz 284.1 Kb (ftp)(http) TXT
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