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Series GSE133691 Query DataSets for GSE133691
Status Public on Sep 14, 2021
Title Gene expression profiles of Lgr5-GFP high and Lgr5-GFP low cells in small intestines of Itgb7+/+ Lgr5-GFP mice and Itgb7-/- Lgr5-GFP mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Intestinal stem cell (ISC) differentiation is regulated precisely by a niche in the crypt, where lymphocytes may interact with stem and transient amplifying (TA) cells. However, whether and how lymphocyte-stem/TA cell contact affects ISC differentiation is largely unknown. Here, we uncover a novel role of T cell-stem/TA cell contact in ISC fate decisions. We show that intestinal lymphocyte depletion results in skewed ISC differentiation in mice, which can be rescued by T cell transfer. Mechanistically, integrin αEβ7 expressed on T cells binds to E-cadherin on ISCs and TA cells, triggering E-cadherin endocytosis and the consequent Wnt and Notch signaling alterations. Blocking αEβ7—E-cadherin adhesion suppresses Wnt signaling and promotes Notch signaling in ISCs and TA cells, leading to defective ISC differentiation. Thus, αEβ7+ T cells regulate ISC differentiation at single-cell level through cell-cell contact-mediated αEβ7—E-cadherin adhesion signaling, highlighting a critical role of the T cell-stem/TA cell contact in maintaining intestinal homeostasis.
Overall design Gene expression profiles of Lgr5-GFP high (stem) and Lgr5-GFP low ( transient amplifying) cells in small intestines of Itgb7+/+ Lgr5-GFP mice and Itgb7-/- Lgr5-GFP mice
Contributor(s) Chen J
Citation(s) 34518654
Submission date Jul 02, 2019
Last update date Dec 19, 2021
Contact name Chen Shiyang
Organization name Shanghai Institute of Biochemistry and Cell Biology (CAS)
Lab Jianfeng Chen's Lab
Street address Room 413, New biochemistry building, YueYang Road
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (20)
GSM3916296 Lgr5high-WT-1
GSM3916297 Lgr5high-WT-3
GSM3916299 Lgr5high-WT-6
BioProject PRJNA552264
SRA SRP212758

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Supplementary file Size Download File type/resource
GSE133691_Lgr5-GFP_high_Itgb7_KO_vs_WT_count.txt.gz 294.1 Kb (ftp)(http) TXT
GSE133691_Lgr5-GFP_low_Itgb7_KO_vs_WT_count.txt.gz 291.0 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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