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Series GSE133647 Query DataSets for GSE133647
Status Public on Sep 30, 2021
Title Transcriptome analysis of hair follcile stem cell quiescence
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Hair follicle stem cells(HFSCs) can transition between active and quiescent stages during normal hair cycle or would healing. Transcriptional regulation has been show to regulate the transition. Previous studies have shown that two transcription factor, Foxc1 and Nfatc1, can help maintain the stem cells in quiescent state, the deletion of either one could lead to precocious activation. Here, we collect the hair follicle stem cell at postnatal day 30 from both control mice, Nfatc1 knockout mice, Foxc1 knockout mice(downloaded) and Foxc1/Nfatc1 double knockout mice to study the transcriptional regulation network of HFSCs quiescence.
Overall design Poly(A)-enriched transcriptome RNA-seq on HFSCs isolated at anagen stage(P30) of hair cycle. For K14Cre Nfatc1 control and cKO mice ,HFSCs were isolated using FACS sorting enriching DAPIneg, K14-H2BGFPhi, Sca1lo, α6hi and CD34hi cells. For K15CrePR mouse line, mice were treated with tomaxifen for 6days for induction and dKO HFSCs were sorted enriching DAPIneg, K14-H2BGFPhi , tdtomatohi, Sca1lo and CD34hi cells, control HFSCs were sorted enriching DAPIneg, K14-H2BGFPhi, Sca1low and CD34hi cells.
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Contributor(s) Zhang C
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Submission date Jul 01, 2019
Last update date Nov 02, 2021
Contact name Chi Zhang
Organization name University of Colorado Boulder
Department MCDB
Lab Yi Rui Lab
Street address 347 UCB
City Boulder
State/province Colorado
ZIP/Postal code 80309
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM3914155 Nfatc1 cKO1
GSM3914156 Nfatc1 cKO2
GSM3914157 Nfatc1 Ctrl1
This SubSeries is part of SuperSeries:
GSE133648 Escape of hair follicle stem cells from a defective niche
BioProject PRJNA552097
SRA SRP212732

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133647_RAW.tar 830.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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