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Series GSE133555 Query DataSets for GSE133555
Status Public on Jun 22, 2020
Title RNA seq to compare gene expression profiles between Npm1 WT and Npm1 knockdown cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Mouse KrasG12D/P53-/-(KP) lung tumor cells were transfected with pLKO.1-Tet on-shNpm1-3(N3), pLKO.1-Tet on-shNpm1-4(N4) or pLKO.1-Tet on-shNpm1-5 (N5) and then selected by using 2ug/ml puromycin, to establish the 3 stable cell lines. These 3 stable cell lines (N3, N4 or N5) were treated with 100ng/ml Doxycline and then the total RNA of the treated and untreated cells were harvested for RNA seq.
 
Overall design Examination of differential gene expression profils in KP-shNpm1-3 (N3), KP-shNpm1-4 (N4) and KP-shNpm1-5 (N5) cells with or without Doxycycline treatment
 
Contributor(s) Li F, Khodadadi-Jamayran A, Wong K
Citation(s) 32646968
Submission date Jun 30, 2019
Last update date Sep 21, 2020
Contact name Alireza Khodadadi-Jamayran
Organization name New York University, NYU Langone Medical Center
Department Division of Advanced Research Technologies (DART)
Lab Applied Bioinformatics Laboratories (ABL)
Street address 550 1st Ave, MSB 304
City New York City
State/province NY
ZIP/Postal code 10016
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM3912006 KP-N3-Dox
GSM3912007 KP-N3
GSM3912008 KP-N4-Dox
Relations
BioProject PRJNA551883
SRA SRP212657

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133555_RAW.tar 650.9 Mb (http)(custom) TAR (of BW, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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