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Series GSE133473 Query DataSets for GSE133473
Status Public on May 22, 2020
Title Requirement for Cleavage Factor IIm in the Control of Alternative Polyadenylation in Breast Cancer Cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary This experimental series concerns the position bias and extent of 3'-end choice in MCF7 cells after knock-down of the CFII subunits PCF11 and CLP1 in MCF7. Loss of either subunit results in 3'-lengthening by APA. Importantly this lengthening is toward a more 'normal-like' distribution. MCF7 cells show a native 3'-end distribution favoring short internal adenylation sites compared to MDAMD231 cells. The difference between the two cell lines might be driven by a higher expression of the exosome in MDAMD231 cells.
Overall design Analysis of 3'-focused PAT-seq data in human breast cancer cell-lines (MCF7 vs MDAMB231) and after siRNA depletion of the CFII subunits (CLP1 and PCF11)
Contributor(s) Dichtl B, Turner R, Beilharz TH
Citation(s) 32295865
Submission date Jun 27, 2019
Last update date May 22, 2020
Contact name Rachael Turner
Organization name Monash University
Department Biochemistry and Molecular Biology
Street address Wellington Road
City Clayton
State/province Victoria
ZIP/Postal code 3800
Country Australia
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (11)
GSM3909831 MCF7_R1
GSM3909832 MCF7_R2
GSM3909833 MDAMD231_R1
BioProject PRJNA551515
SRA SRP212229

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Supplementary file Size Download File type/resource
GSE133473_count.csv.gz 601.3 Kb (ftp)(http) CSV
GSE133473_gene.csv.gz 2.2 Mb (ftp)(http) CSV
GSE133473_tail-count.csv.gz 553.5 Kb (ftp)(http) CSV
GSE133473_tail.csv.gz 1.1 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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