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Series GSE133454 Query DataSets for GSE133454
Status Public on Jun 27, 2022
Title Single-cell gene expression reveals the stage-specific roles of TCF1 in T cell development and T cell malignancy
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary T cell factor 1 (TCF1; encoded by Tcf7) plays a critical role in T cell development and functions as a tumor suppressor by repressing the expression of LEF1 in the thymus. Here we reported the stage-specific regulation of TCF1 in early T cell differentiation and T cell malignancy despite the broad understand on the functions of TCF1. To illustrate the stage-specific functions of Tcf7 in T cell development and tumorigenesis, we applied single-cell transcriptional analysis to identify the precise trajectory of gene expression changes between the surface receptor-defined stages of T cell development.
Overall design To systematically investigate the gene expression pattern in WT and stage-specific deletion of Tcf7, we sequenced 768 single cells from four genotype mice (WT, Tcf7-/-, Tcf7fl/flVav-cre, Tcf7fl/flLck-cre). The cell type of WT is DN1 (96 single cell) and DN2-3 (96 single cell). The cell type of Tcf7-/- is DN1 (96 single cell) and DN2-3 (96 single cell). The cell type of Tcf7fl/flVav-cre is DN1 (96 single cell) and DN2-3 (96 single cell). The cell type of Tcf7fl/flLck-cre is DN2-3 (192 single cell).
Contributor(s) Yu S, Wang F
Citation(s) 32868912
Submission date Jun 27, 2019
Last update date Sep 26, 2022
Contact name Shuyang Yu
Organization name China Agricultural University
Department College of Biological Sciences
Street address 2 Yuanmingyuan West Road
City Beijing
ZIP/Postal code 100193
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (768)
GSM3908775 WT-DN1_BC_10
GSM3908776 WT-DN1_BC_11
GSM3908777 WT-DN1_BC_12
BioProject PRJNA551482
SRA SRP212213

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133454_barcode_information.xlsx 34.9 Kb (ftp)(http) XLSX
GSE133454_count_matrix.csv.gz 3.1 Mb (ftp)(http) CSV
GSE133454_expression_matrix_log2TPM_10+1.csv.gz 11.8 Mb (ftp)(http) CSV
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Processed data are available on Series record

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