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Series GSE133412 Query DataSets for GSE133412
Status Public on Sep 11, 2019
Title PRC2.1 and PRC2.2 synergize to co-ordinate H3K27 tri-methylation. 
Organisms Drosophila melanogaster; Homo sapiens; Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Polycomb Repressive Complex 2 (PRC2) is composed of EED, SUZ12, and EZH1/2 and mediates mono-, di- and tri-methylation of Histone H3 at Lysine 27. While at least two subcomplexes exist, defined by their specific accessory proteins, termed PRC2.1 (Polycomb-like proteins 1-3, EPOP and PALI1/2) and PRC2.2 (AEBP2 and JARID2), little is known about their differential functions. Here, we show that PRC2.1 and PRC2.2 share the majority of target genes in mouse embryonic stem cells (ESCs). The loss of all three Polycomb-like proteins is sufficient to destabilise and evict PRC2.1 from chromatin, but also leads to reduced PRC2.2 and H3K27me3 at most Polycomb domains. However, the combined loss of PRC2.1 and PRC2.2 is necessary to completely remove H3K27me3 at broad Polycomb domains such as the Hox loci. Our data support a model in which the specific accessory proteins within PRC2.1 and PRC2.2 co-operate to direct and maintain H3K27me3, via both synergistic and independent mechanisms.
Overall design Quantitative ChIP-Rx analysis of Pcl1-3 WT EV, Pcl1-3 TKO EV and Pcl1-3/Jarid2 c12 QKO mouse embryonic stem cells. ChIPs include a 10% spike in of human chromatin (NT2 cell line). 25 samples are included.
Contributor(s) Healy E, Mucha M, Fitzpatrick DJ, Glancy E, Conway E, Neikes HK, Monger C, Van Mierlo G, Baltissen MP, Koseki Y, Vermuelen M, Koseki H, Bracken AP
Citation(s) 31521505
Submission date Jun 27, 2019
Last update date Mar 02, 2020
Contact name Craig Monger
Organization name Trinity College Dublin
Department Smurfit Institute of Genetics
Street address College Green
City Dublin 2
State/province Ireland
ZIP/Postal code EIRE
Country Ireland
Platforms (2)
GPL19415 Illumina NextSeq 500 (Homo sapiens; Mus musculus)
GPL25537 Illumina NextSeq 500 (Drosophila melanogaster; Mus musculus)
Samples (37)
GSM3908331 WT_Jarid2
GSM3908332 TKO_Jarid2
GSM3908333 QKO_Jarid2
This SubSeries is part of SuperSeries:
GSE127121 Variant PRC2.1 and PRC2.2 co-operate to direct H3K27 methylations in ESCs
BioProject PRJNA551426
SRA SRP212177

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133412_RAW.tar 1.9 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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