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| Status |
Public on Dec 31, 2021 |
| Title |
A Congenital Anemia Dissociates the Pleiotropic Functions of Master Transcription Factor GATA1 [G1E_ATACseq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Master regulators, such as the hematopoietic transcription factor GATA1, have numerous roles in lineage commitment and differentiation. While human GATA1 mutations result in several blood diseases, all characterized mutations act relatively early to impair hematopoietic differentiation. Here, we describe a distinct form of hemolytic anemia involving impaired terminal erythropoiesis with a reduced lifespan of circulating red blood cells. We show that this unique blood disorder results from mutations in a poorly characterized and intrinsically disordered C-terminal region of GATA1.
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| Overall design |
Murine G1E cells were transduced with the HMD lentiviral vector encoding human wildtype GATA1 or point mutants p.R307C or p.R307H
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| Contributor(s) |
Ludwig L, Lareau C |
| Citation missing |
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| |
| Submission date |
Jun 27, 2019 |
| Last update date |
Jan 01, 2022 |
| Contact name |
Caleb Lareau |
| E-mail(s) |
lareauc@mskcc.org
|
| Organization name |
Memorial Sloan Kettering
|
| Street address |
417 E 68th St, Zuckerman - ZRC 1132
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE133417 |
A Congenital Anemia Dissociates the Pleiotropic Functions of Master Transcription Factor GATA1 |
|
| Relations |
| BioProject |
PRJNA551412 |
| SRA |
SRP212172 |
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