|
| Status |
Public on Aug 08, 2019 |
| Title |
Exploring genetic interaction manifolds constructed from rich phenotypes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Rich phenotyping using single-cell RNA sequencing reveals principles and mechanisms of genetic interactions.
|
| |
|
| Overall design |
Single-cell, pooled CRISPR screening experiment comparing the transcriptional effects of overexpressing genes alone or in combination
|
| |
|
| Contributor(s) |
Norman TM, Horlbeck MA, Gilbert LA, Weissman JS |
| Citation(s) |
31395745 |
| Submission date |
Jun 26, 2019 |
| Last update date |
Apr 23, 2020 |
| Contact name |
Thomas Norman |
| E-mail(s) |
thomas.norman@ucsf.edu
|
| Organization name |
University of California, San Francisco
|
| Department |
CMP
|
| Lab |
Weissman
|
| Street address |
Biochemistry Box 2542, 1700 4th St QB3 Rm 404
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94103 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (16)
|
| GSM3906020 |
sgRNA perturb-seq experiment (gemgroup 1) |
| GSM3906021 |
sgRNA perturb-seq experiment (gemgroup 2) |
| GSM3906022 |
sgRNA perturb-seq experiment (gemgroup 3) |
| GSM3906023 |
sgRNA perturb-seq experiment (gemgroup 4) |
| GSM3906024 |
sgRNA perturb-seq experiment (gemgroup 5) |
| GSM3906025 |
sgRNA perturb-seq experiment (gemgroup 6) |
| GSM3906026 |
sgRNA perturb-seq experiment (gemgroup 7) |
| GSM3906027 |
sgRNA perturb-seq experiment (gemgroup 8) |
| GSM3906028 |
Barcodes identifying perturbations (gemgroup 1) |
| GSM3906029 |
Barcodes identifying perturbations (gemgroup 2) |
| GSM3906030 |
Barcodes identifying perturbations (gemgroup 3) |
| GSM3906031 |
Barcodes identifying perturbations (gemgroup 4) |
| GSM3906032 |
Barcodes identifying perturbations (gemgroup 5) |
| GSM3906033 |
Barcodes identifying perturbations (gemgroup 6) |
| GSM3906034 |
Barcodes identifying perturbations (gemgroup 7) |
| GSM3906035 |
Barcodes identifying perturbations (gemgroup 8) |
|
| Relations |
| BioProject |
PRJNA551220 |
| SRA |
SRP212114 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133344_RAW.tar |
10.1 Gb |
(http)(custom) |
TAR (of H5) |
| GSE133344_filtered_barcodes.tsv.gz |
419.9 Kb |
(ftp)(http) |
TSV |
| GSE133344_filtered_cell_identities.csv.gz |
1.9 Mb |
(ftp)(http) |
CSV |
| GSE133344_filtered_genes.tsv.gz |
258.6 Kb |
(ftp)(http) |
TSV |
| GSE133344_filtered_matrix.mtx.gz |
1.1 Gb |
(ftp)(http) |
MTX |
| GSE133344_raw_barcodes.tsv.gz |
17.4 Mb |
(ftp)(http) |
TSV |
| GSE133344_raw_cell_identities.csv.gz |
2.1 Mb |
(ftp)(http) |
CSV |
| GSE133344_raw_genes.tsv.gz |
258.6 Kb |
(ftp)(http) |
TSV |
| GSE133344_raw_matrix.mtx.gz |
1.3 Gb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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