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Series GSE133326 Query DataSets for GSE133326
Status Public on Jun 27, 2019
Title Global gene networks in canine peripheral blood mononuclear cells infected with Mycobacterium avium subsp.hominissuis
Organism Canis lupus familiaris
Experiment type Expression profiling by high throughput sequencing
Summary An opportunistic intracellular pathogen Mycobacterium avium subsp. hominissuis, a member of the nontuberculous mycobacteria (NTM) cluster, causes respiratory disease in immunosuppressed hosts. In particular, infected companion dogs are a potential role to transmit the agent to children or immunosuppressed people. The purpose of this study is to investigate a host-M. avium hominissuis interactome in canine PBMCs during the infection.
Overall design Peripheral blood mononuclear cells were isolated from buffy coats of six healthy beagle dogs by density gradient centrifugation. PBMCs were stimulated in vitro with M. avium hominissuis strain 104. Total RNAs were extracted from the cells at 2h, 6h, 12h, and 24h after infection. The construction of library was performed using QuantSeq 3’mRNA-Seq Library Prep Kit. High-throughput sequencing was performed as single-end 75 sequencing using NextSeq 500.
Contributor(s) Kim S, Yoo H
Citation(s) 33520738
Submission date Jun 26, 2019
Last update date Feb 08, 2021
Contact name Suji Kim
Organization name Seoul National University
Department Veterinary Medicine
Lab Infectious Disease
Street address 1, Gwanak-ro
City Seoul
State/province Gwanak-gu
ZIP/Postal code ASI/KR/KS013
Country South Korea
Platforms (1)
GPL21400 Illumina NextSeq 500 (Canis lupus familiaris)
Samples (16)
GSM3905777 PBMCs, 0HC1
GSM3905778 PBMCs, 0HC2
GSM3905779 PBMCs, 0HM1
BioProject PRJNA551125
SRA SRP212100

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133326_RAW.tar 2.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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