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Series GSE133274 Query DataSets for GSE133274
Status Public on Jun 28, 2019
Title Effects of transcription on genome - nuclear lamina interactions: Repli-seq data
Organism Homo sapiens
Experiment type Other
Summary In mammalian nuclei, transcriptionally active genomic regions tend to localize to the interior of the nucleus while inactive regions are often located at at the nuclear lamina. In this study we activated specific genes in lamina-associated domains by TALE-VP64 or CRISPRa-mediated activation. We also reduced transcription of individual genes by knockout of promoter/enhancer regions, or by insertion of a transcription termination sequence. In each case we generated genome-wide DamID maps to determine changes in nuclear lamina interactions, and in selected cases we also generated Repli-seq maps and/or RNAseq data.
Overall design Transcription of individual genes was manipulated in human RPE-1 cells by CRISPRa. Changes in replication timing were determined by Repli-seq.
Contributor(s) Brueckner L, van Schaik T, Leemans C, Sima J, Zhao PA, Gilbert DM, van Steensel B
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Submission date Jun 25, 2019
Last update date Jun 30, 2019
Contact name Bas van Steensel
Phone + 31 20 512 2040
Fax +31 20 669 1383
Organization name Netherlands Cancer Institute
Department division of Molecular Biology
Lab van Steensel group
Street address Plesmanlaan 121
City Amsterdam
ZIP/Postal code 1066 CX
Country Netherlands
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (24)
GSM3908057 Repli-seq, early, RPE, CRISPRa, ABCB1, rep1
GSM3908058 Repli-seq, early, RPE, CRISPRa, ABCB1, rep2
GSM3908059 Repli-seq, early, RPE, CRISPRa, ADAM22, rep1
This SubSeries is part of SuperSeries:
GSE133275 Effects of transcription on genome - nuclear lamina interactions
BioProject PRJNA551389
SRA SRP212160

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Supplementary file Size Download File type/resource
GSE133274_RAW.tar 1.1 Gb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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