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Series GSE133251 Query DataSets for GSE133251
Status Public on Sep 05, 2020
Title The effect of YAP/TAZ on the expression of the heat shock transcriptome
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We found that heat stress induces a strong and rapid YAP dephosphorylation and activation. To determin what is the physiological functions of YAP in response to heat stress, we performed RNA-SEQ analysis on siCon and siYAP/TAZ knockdown tumor cells with or without heat shock. Results indicated that 95% of heat inducible genes is blocked (83% completely and 12% partially) by YAP/TAZ knockdown in the B16-OVA melanoma cells whereas only 5% of heat inducible genes were unaffected, supporting a previously unrecognized prominent role of YAP/TAZ in the expression of the heat shock transcriptome and cell survival.
Overall design siCon and siYAP/TAZ knockdown cells with or without heat shock, in triplicate.
Contributor(s) Luo M, Shao B
Citation(s) 33199845
Submission date Jun 24, 2019
Last update date Jan 05, 2021
Contact name Min Luo
Organization name SICHUAN UNIVERSITY
Street address NO.17, the third section of South Renmin Road
State/province SICHUAN
ZIP/Postal code 610041
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (11)
GSM3904129 WT_NT_1
GSM3904130 WT_NT_2
GSM3904131 WT_NT_3
BioProject PRJNA551001
SRA SRP212072

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Supplementary file Size Download File type/resource
GSE133251_gene_fpkm_matrix.csv.gz 1.4 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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