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Series GSE133216 Query DataSets for GSE133216
Status Public on Feb 06, 2020
Title The effects of METTL3 on BAT development and thermogenesis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goal of this study is to compare transcriptome profilings of interscapular brown adipose tissue (iBAT) from BAT-specific METTL3 knockout and control mice

Methods: iBATs were collected from METTL3 BKO and f/f mice at 8 weeks old, and total RNA was isolated using TriPure. RNA samples were pooled from iBATs of METTL3 BKO and f/f mice (n=4 for each group). RNA-seq was performed by using Illumina NovaSeq 6000 platform. Paired-end clean reads were aligned to the mouse reference genome (Ensemble_GRCm38.90) with TopHat (version 2.0.12), and the aligned reads were used to quantify mRNA expression by using HTSeq-count (version 0.6.1).

Conclusion:Our study represents the first detailed analysis of iBAT transcriptomes from METTL3 BKO and f/f mice, generated by RNA-seq technology. The RNA-seq analysis showed that 530 genes were down-regulated and 1552 genes were up-regulated in the iBAT of METTL3 BKO mice. GO analysis showed that the down-regulated genes were primarily related to developmental maturation, respiratory electron transport chain, adaptive thermogenesis, and energy deprivation by oxidation of organic compounds whereas the up-regulated genes were associated with muscle system process and muscle cell development.
Overall design RNA samples were pooled from iBATs of METTL3 BKO and f/f mice at 8 weeks old (n=4 for each group). mRNA profiles of METTL3 BKO and f/f iBATs were generated by deep sequencing, in a single test, using Illumina NovaSeq 6000 platform.
Contributor(s) Chen Z, Wang Y
Citation(s) 32245957
Submission date Jun 24, 2019
Last update date Apr 13, 2020
Contact name Zheng Chen
Phone +8645186402029
Organization name Harbin Institute of Technology
Street address 92 Xidazhijie Harbin Institute of Technology
City Harbin
State/province Heilongjiang
ZIP/Postal code 150001
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM3902459 f/f
GSM3902460 BKO
BioProject PRJNA550401
SRA SRP211828

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Supplementary file Size Download File type/resource
GSE133216_RAW.tar 2.1 Mb (http)(custom) TAR (of XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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