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| Status |
Public on Feb 06, 2020 |
| Title |
The effects of METTL3 on BAT development and thermogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goal of this study is to compare transcriptome profilings of interscapular brown adipose tissue (iBAT) from BAT-specific METTL3 knockout and control mice
Methods: iBATs were collected from METTL3 BKO and f/f mice at 8 weeks old, and total RNA was isolated using TriPure. RNA samples were pooled from iBATs of METTL3 BKO and f/f mice (n=4 for each group). RNA-seq was performed by using Illumina NovaSeq 6000 platform. Paired-end clean reads were aligned to the mouse reference genome (Ensemble_GRCm38.90) with TopHat (version 2.0.12), and the aligned reads were used to quantify mRNA expression by using HTSeq-count (version 0.6.1).
Conclusion:Our study represents the first detailed analysis of iBAT transcriptomes from METTL3 BKO and f/f mice, generated by RNA-seq technology. The RNA-seq analysis showed that 530 genes were down-regulated and 1552 genes were up-regulated in the iBAT of METTL3 BKO mice. GO analysis showed that the down-regulated genes were primarily related to developmental maturation, respiratory electron transport chain, adaptive thermogenesis, and energy deprivation by oxidation of organic compounds whereas the up-regulated genes were associated with muscle system process and muscle cell development.
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| Overall design |
RNA samples were pooled from iBATs of METTL3 BKO and f/f mice at 8 weeks old (n=4 for each group). mRNA profiles of METTL3 BKO and f/f iBATs were generated by deep sequencing, in a single test, using Illumina NovaSeq 6000 platform.
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| Contributor(s) |
Chen Z, Wang Y |
| Citation(s) |
32245957 |
| Submission date |
Jun 24, 2019 |
| Last update date |
Apr 13, 2020 |
| Contact name |
Zheng Chen |
| E-mail(s) |
chenzheng@hit.edu.cn
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| Phone |
+8645186402029
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| Organization name |
Harbin Institute of Technology
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| Street address |
92 Xidazhijie Harbin Institute of Technology
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| City |
Harbin |
| State/province |
Heilongjiang |
| ZIP/Postal code |
150001 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA550401 |
| SRA |
SRP211828 |
