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Series GSE133097 Query DataSets for GSE133097
Status Public on Aug 11, 2020
Title Identification of ALPPL2 as a novel specific and functional surface marker for naïve pluripotency
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We report ALPPL2 as a prominently specific and functional surface marker for naïve pluripotency. By performing RNA-seq analysis of TJ-1# and iCRISPR nESCs and pESCs, APG+ cells at different time points during naïve reprogramming, we validate ALPPL2’s specificity for naïve pluripotent state at transcriptional level. Deep analysis of the transcriptome differences between Wild Type (WT), ALPPL2 knockdown (AKD) cells via RNA-seq, Wild Type (WT), ALPPL2 knockout (AKO) and IGFBP1 knockout (IKO) individual colonies in detail via Smart-seq, and performing RNA immunoprecipitation (RIP) using IGF2BP1 antibody in nESCs followed by RNA-seq or qPCR analysis, we identify that ALPPL2 is essential for both establishment and maintenance of naïve pluripotency via interacting with IGF2BP1 to stabilize TFCP2L1 and STAT3 mRNA level.
Overall design Examination of mRNA profiles of WT, ALPPL2 KO, ALPPL2 KD, IGF2BP1 KO and IGF2BP1 enrichment by deep sequencing.
Contributor(s) Bi Y, Zhang Y, Wang Y
Citation(s) 32187559
Submission date Jun 20, 2019
Last update date Apr 20, 2022
Contact name Bi Yan
Phone 18916058932
Organization name Tongji university
Street address Siping Road 1239
City Shanghai
ZIP/Postal code 200092
Country China
Platforms (2)
GPL20795 HiSeq X Ten (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (74)
GSM4721268 293T AOE rep1
GSM4721269 293T AOE rep2
GSM4721270 293T WT rep1
BioProject PRJNA656543
SRA SRP277076

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133097_RAW.tar 25.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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