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Series GSE133085 Query DataSets for GSE133085
Status Public on Aug 06, 2020
Title Two distinct functional axes of positive feedback-enforced PRC2 recruitment in mouse embryonic stem cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Polycomb Repressive Complex 2 (PRC2) plays an essential role in gene repression during development, catalysing H3 lysine 27 trimethylation (H3K27me3). MTF2 in the PRC2.1 sub-complex, and JARID2 in PRC2.2, are central in core PRC2 recruitment to target genes in mouse embryonic stem cells (mESCs). To investigate how PRC2.1 and PRC2.2 cooperate, we combined Polycomb mutant mESCs with chemical inhibition of binding to H3K27me3. We find that PRC2.1 and PRC2.2 mediate two distinct paths for recruitment, which are mutually reinforced. Whereas PRC2.1 recruitment is mediated by MTF2 binding to DNA, JARID2-containing PRC2.2 recruitment is more dependent on PRC1. Both recruitment axes are supported by core subunit EED binding to H3K27me3, but EED inhibition exhibits a more pronounced effect in Jarid2 null cells. Finally, we show that PRC1 and PRC2 enhance reciprocal binding. Together, these data disentangle the interdependent interactions that are important for PRC2 recruitment.
Overall design ChIP-seq for Mtf2, Ezh2, Jarid2, Ring1b, H3K27me3 and input for reference performed in WT mESC or mESC mutant for Mtf2, Jarid2, EED, Ring1a/b with or without the PRC2 inhibitor EED226 or the PRC1 inhibitor MG32. Validation of quantification with spike-In ChIP for Ezh2 in WT, EED KO and Mtf2 GT lines. Part of the data are re-analized from Perino et al, 2018, Nat. Gen.
Contributor(s) Perino M, van Mierlo G, Loh C, Wardle S, Zijlmans D, Marks H, Veenstra GJ
Citation(s) 32763159
Submission date Jun 20, 2019
Last update date Nov 05, 2020
Contact name Gert Jan Veenstra
Phone +31 24 3610541
Organization name Radboud University
Department Molecular Developmental Biology
Street address Geert Grooteplein 28
City Nijmegen
State/province Not US or Canada
ZIP/Postal code 6525 GA
Country Netherlands
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (69)
GSM3899521 E14_FCS_226_Ezh2.mapUnique
GSM3899522 E14_FCS_226_Ezh2_A.mapUnique
GSM3899523 E14_FCS_226_Ezh2_B.mapUnique
BioProject PRJNA549925
SRA SRP202007

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Supplementary file Size Download File type/resource
GSE133085_RAW.tar 2.5 Gb (http)(custom) TAR (of BAM)
GSE133085_processed_data.bed.tar.gz 110.5 Kb (ftp)(http) TAR 6.7 Gb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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