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Series GSE132952 Query DataSets for GSE132952
Status Public on Aug 19, 2019
Title Methylation and gene expression data from Dnmt3b+/+, Dnmt3b-/- and Dnmt3bCI/CI embryos harvested at E11.5
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Dnmt3b is a DNA methyltransferase, an enzyme which methylates genomic DNA, contributes to genomic stability and transcriptional regulation. Inactivation of Dnmt3b in mice results in embryonic lethality at E13.5. It is however unclear which activities are responsible for this phenomenon. Here, we analyzed methylation and gene expression in Dnmt3b+/+ (WT), Dnmt3b-/- (3bKO) and Dnmt3bCI/CI (3bKI) embryos at E11.5.
Overall design We performed RRBS and RNA-seq analysis in whole embryos harvested at E11.5. RRBS experiments were performed in biological duplicates, RNA-seq experiments in triplicates. In addition, we performed RRBS in Dnmt3a-/- Dnmt3b-/- lymphoma cell line overexpressing Dnmt3b WT and CI.
Contributor(s) Nowialis P, Lopusna K, Opavska J, Haney SL, Abraham A, Sheng P, Riva A, Natarajan A, Guryanova O, Simpson M, Hlady R, Xie M, Opavsky R
Citation(s) 31558711
Submission date Jun 18, 2019
Last update date Nov 18, 2019
Contact name Rene Opavsky
Phone 3522948599
Organization name University of FLORIDA
Department Anatomy and cell biology
Street address 2033 Mowry rd, CGRC r..265
City Gainesville
State/province FL
ZIP/Postal code 32610
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (18)
GSM3897441 RRBS WT 1
GSM3897442 RRBS WT 2
GSM3897443 RRBS 3bKO 1
BioProject PRJNA549518
SRA SRP201807

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Supplementary file Size Download File type/resource
GSE132952_RAW.tar 94.7 Mb (http)(custom) TAR (of BIGWIG)
GSE132952_RNAseq_FPKM.txt.gz 2.5 Mb (ftp)(http) TXT
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Processed data provided as supplementary file
Processed data are available on Series record
Raw data are available in SRA

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