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Series GSE132902 Query DataSets for GSE132902
Status Public on Jun 18, 2022
Title CDK5 inhibition abrogates TNBC stem-cell property and enhances anti-PD-1 therapy
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Triple-negative breast cancer (TNBC) is a highly aggressive subtype of breast cancer that shows high infiltration of cancer stem cells (CSCs), which correlates with poor clinical outcome. Here, we have demonstrated that an aberrant activation of CDK5/pho-PPARg axis associated with TNBC progression closely. CDK5 blockade sufficiently abrogates stemness transformation of TNBC cells, resulting in a significant inhibition of tumor metastatic progression. Moreover, CDK5 inhibitor Rosc attenuates CD44v+ BCSCs, hereby reversing immunosuppressive microenvironment and enhancing anti-PD-1 effects on TNBC. Mechanistically, CDK5/pho-PPARgaxis modulates the ESRP1 degradation via E3 ligase-like activity, leading to CD44 variant (CD44v) expression. Our finding indicates that CDK5 blockade could be a potent strategy to target CSCs in TNBC, and to increase the response to PD-1 blockade in TNBC therapy.
Overall design mRNA profiles of 4T1 mouse TNBC cells with or without treatment of CDK5 inhibitor, Rosc, were generated by deep sequencing, in triplicate.
Contributor(s) Bei Y, Cheng N, Chen T, Shu Y, Yang Y, Yang N, Zhou X, Liu B, Wei J, Liu Q, Zheng W, Zhang W, Su H, Zhu W, Ji J, Shen P
Citation(s) 33240752
Submission date Jun 18, 2019
Last update date Nov 04, 2022
Contact name Yuncheng Bei
Phone 8618205187781
Organization name Nanjing Drum Tower Hospital
Department Oncology
Lab Baorui Liu
Street address Zhongshan Road No.321
City Nanjing
State/province Jiangsu
ZIP/Postal code 210046
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM3895945 C1: vehicle
GSM3895946 C2: vehicle
GSM3895947 C3: vehicle
BioProject PRJNA549437
SRA SRP201745

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Supplementary file Size Download File type/resource
GSE132902_RAW.tar 12.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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