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Series GSE132847 Query DataSets for GSE132847
Status Public on Jun 18, 2019
Title Transcriptional profile analysis of uninfected or VSV-infected scrambled siRNA (siControl) and DDX23 knockdown (siDDX23) A549 cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary DExD/H-box helicases play essential roles in RNA metabolism, and emerging data suggest that they have additional functions in antiviral immunity across species. However, little is known about this evolutionarily conserved family in antiviral responses in lower species. Here, by isolation of poly(I:C)-binding proteins in amphioxus, an extant basal chordate, we found that DExD/H-box helicases DHX9, DHX15 and DDX23 to be responsible for cytoplasmic dsRNA detection in amphioxus. Since the antiviral roles of DDX23 have not been characterized in mammals, we performed further poly(I:C) pull down assays and found human DDX23 bind to LMW poly(I:C) through its N-terminal region, suggesting that DDX23 is an evolutionarily conserved dsRNA sensor. Knockdown of human DDX23 enhanced replication of VSV and reduced activation of the NF-κB and IRF3. Moreover, when stimulated with poly(I:C) or VSV, human DDX23 translocated from nucleus to cytoplasm and formed complexes with TRIF or MAVS to initiate downstream signaling. Collectively, this comparative immunological study not only defined DDX23 as an emerging nuclear pattern recognition receptor (PRR) for innate sensing of RNA virus, but also extended the essential role of the DExD/H helicase family in viral RNA sensing from mammals to basal chordate.
Overall design The RNA sequencing studies were performed with A549 cells transfected with scrambled siRNA (siControl ) or siDDX23 targeting DDX23 gene for three days following 12 hours infection with VSV (MOI=3) or uninfected. Total RNA was isolated using TRIZOL Reagent (Invitrogen). cDNA library was constructed using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and then sequenced with HiSeq 2000 system (Illumina Inc.). The expression of transcripts was quantified as reads per kilobase of exon model per million mapped reads (RPKM). Subsequently, gene enrichment analysis was conducted using the DAVID tool.
Contributor(s) Yuan S, Ruan J
Citation(s) 31620127
Ruan J, Cao Y, Ling T, Li P, Wu S, Peng D, Wang Y, Jia X, Chen S, Xu A and Yuan S (2019) DDX23, an Evolutionary Conserved dsRNA Sensor, Participates in Innate Antiviral Responses by Pairing With TRIF or MAVS. Front. Immunol. 10:2202. doi: 10.3389/fimmu.2019.02202.
Submission date Jun 17, 2019
Last update date Oct 21, 2019
Contact name Shaochun Yuan
Organization name Sun yat-sen university
Street address 132 Waihuan East Road
City Guangzhou
ZIP/Postal code 510006
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM3895060 siControl A549
GSM3895061 siDDX23 A549
GSM3895062 siControl A549 VSV
BioProject PRJNA549265
SRA SRP201617

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Supplementary file Size Download File type/resource
GSE132847_All_gene_rpkm.list.xls.gz 1.2 Mb (ftp)(http) XLS
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