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| Status |
Public on Aug 06, 2019 |
| Title |
Identification of a T follicular helper subset that drives anaphylactic IgE |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report a rare population of IL-13-producing Tfh cells that were present with high-affinity IgE to allergens. These “Tfh13” cells have an unusual cytokine profile (IL-13hiIL-4hiIL-5hiIL-21lo), co-express BCL6 and GATA3 and were required for production of high- but not low-affinity IgE and accordingly, allergen-induced anaphylaxis.
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| Overall design |
Single-cell RNA-seq from 1) Sorted Tfh cells (TCRβ+ CD4+ CD44+ PD-1+ CXCR5+) and 2) Total IL-4 4Get GFP reporter positive CD4 T cells (TCRβ+ CD4+ CD44+ GFP (IL-4)+) cells from day 7 mediastinal LN after Alternaria alternata (10μg) + NP-OVA (25μg) immunization (i.n.).
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| Contributor(s) |
Gowthaman U, Chen JS, Zhang B, Flynn WF, Lu Y, Song W, Joseph J, Gertie JA, Xu L, Collet M, Grassmann J, Simoneau T, Chiang D, Berin MC, Craft JE, Weinstein JS, Williams A, Eisenbarth SC |
| Citation(s) |
31371561 |
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| Submission date |
Jun 16, 2019 |
| Last update date |
Aug 06, 2019 |
| Contact name |
William F Flynn |
| Organization name |
The Jackson Laboratory
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| Street address |
10 Discovery Drive
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| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06032 |
| Country |
USA |
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| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA549081 |
| SRA |
SRP201549 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE132798_RAW.tar |
68.8 Mb |
(http)(custom) |
TAR (of CSV, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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