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Series GSE132771 Query DataSets for GSE132771
Status Public on Jan 30, 2020
Title Single cell RNA-sequencing of normal and fibrotic lungs in mouse and human
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We performed scRNA-seq of cells obtained with a protocol designed to capture all of the collagen-producing cells from normal and fibrotic mouse and human lungs.
Overall design We induced lung fibrosis by treating two Col1a1-GFP reporter mice with bleomycin, and harvested lungs on day 14 after treatment. Two untreated Col1a1-GFP mice were used as control. After tissue dissociation, GFP+ cells and GFP- cells were sorted, and their single cell libraries were generated using 10x Genomics 3' v2 system. For human samples, we sorted lineage (CD31, CD45, EPCAM, CD235a)- cells and all lung cells (CD235a-) from three normal, three IPF, and two scleroderma lungs.
Contributor(s) Tsukui T, Sun KH, Matthay MA, Wolters PJ, Sheppard D
Citation(s) 32317643
Submission date Jun 14, 2019
Last update date Apr 27, 2020
Contact name Tatsuya Tsukui
Organization name UCSF
Department Medicine
Street address 555 Mission Bay Blvd South, 282
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (2)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (24)
GSM3891612 Bleo1_GFPp
GSM3891613 Bleo2_GFPp
GSM3891614 Bleo1_GFPn
BioProject PRJNA548947
SRA SRP201481

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Supplementary file Size Download File type/resource
GSE132771_RAW.tar 526.5 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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