|
| Status |
Public on Jun 14, 2019 |
| Title |
The RNA-Binding Protein DND1 Acts Sequentially as a Negative Regulator of Pluripotency and a Positive Regulator of Epigenetic Modifiers Required for Germ Cell Reprogramming |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Analysis of our transcriptome and RNA immunoprecipitation experiments indicate DND1 acts as a positive regulator of chromatin modifiers in male germ cells, linking RNA binding proteins and epigenetic regulation.
|
| |
|
| Overall design |
Transcriptome timecourse (E12.5, E13.5 and E14.5) comparing muring male germ cells homozygous for Ter mutation in Dnd1 to wild type male germ cells done in conjunction with in vitro work with HEK293 cells transfected with tagged Dnd1 for digestion optimized RNA immunoprecipitation sequencing (DO-RIP-seq) experiments to identify a RNA recognition element for DND1 as well as transcipt targets of this RNA binding protein.
|
| Web link |
https://journals.biologists.com/dev/article/146/19/dev175950/49030/The-RNA-binding-protein-DND1-acts-sequentially-as
|
| |
|
| Contributor(s) |
Capel B, Keene JD, Ruthig VA, Friedersdorf M |
| Citation(s) |
31253634 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| F32 GM129956 |
DND1 Mediated Posttranscriptional Regulation in Murine Prospermatogonia During G1/G0 Arrest |
DUKE UNIVERSITY |
Victor A. Ruthig |
| R01 GM087500 |
Regulation of Germ Cell Pluripotency Through The RNA-Binding Protein, DND1 |
DUKE UNIVERSITY |
Blanche Capel |
| R01 CA157268 |
RNA Dynamics of Transient Macromolecular Complexes in Cancer Cells |
DUKE UNIVERSITY |
Jack D Keene |
|
| |
| Submission date |
Jun 13, 2019 |
| Last update date |
Jan 16, 2024 |
| Contact name |
Victor A Ruthig |
| E-mail(s) |
Victor.Ruthig@CUAnschutz.edu
|
| Organization name |
University of Colorado Anschutz Medical Campus
|
| Department |
Pediatrics, Section of Developmental Biology
|
| Street address |
12800 E 19th Ave
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (28)
|
|
| Relations |
| BioProject |
PRJNA548806 |
| SRA |
SRP201389 |
Less...
More...