|
| Status |
Public on Jun 14, 2019 |
| Title |
Comparison of RhoE and RhoEα regulated pathways |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We identified a new RhoE isoform, RhoEα, and compared the differentially regulated signalings by these two proteins. Both RhoE and RhoEα were first knocked out via CRISPR/Cas9 in HEK 293 cells and HeLa cells (KO cells), followed by RhoE and RhoEα reintroduction (RhoE reintroduction cells and RhoEα reintroduction cells, resepectively). Wild-type cells, KO cells, RhoE reintrocution cells, and RhoEα reintroduction cells were then subjected to RNA-seq analysis.
|
| |
|
| Overall design |
Examination of genes individually regulated by RhoE and RhoEα
|
| |
|
| Contributor(s) |
Dai Y, Ma W, Wang J, Chang J |
| Citation(s) |
33060740 |
| Submission date |
Jun 13, 2019 |
| Last update date |
Oct 27, 2020 |
| Contact name |
Jiang Chang |
| Organization name |
Texas A&M University Institute of Biosciences and Technology
|
| Department |
Center for Translational Cancer Research
|
| Street address |
2121 W. Holcombe Blvd.
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA548801 |
| SRA |
SRP201386 |
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