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Series GSE132695 Query DataSets for GSE132695
Status Public on Jun 14, 2019
Title Prevention of chromatin destabilization by FACT is crucial for malignant transformation [gene expression]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Histone chaperone FACT is commonly expressed and essential for the viability of transformed but not normal cells and its expression levels correlate with poor prognosis in cancer patients. FACT binds several components of nucleosomes and has been viewed as a factor destabilizing nucleosomes to facilitate RNA polymerase passage. To connect FACT’s role in transcription with the viability of tumor cells, we analyzed genome-wide FACT binding to chromatin in conjunction with transcription in mouse and human cells with different degrees of FACT dependence. While genomic distribution and density of FACT correlated with the intensity of transcription, FACT knockout or knockdown was unexpectedly accompanied by the elevation, rather than suppression, of transcription and with the destabilization of chromatin. These data suggest that the real function of FACT is to stabilize and reassemble nucleosomes disturbed by transcription. This function is apparently vital for tumor cells because malignant transformation is accompanied by chromatin destabilization. 
Overall design Mouse skin fibroblasts were isolated from tails of Ssrp1fl/fl;CreERT2+/+ mice. Primary cells were immortalized with p53 suppressive element GSE56 and transformed by GSE56+ HRasV12. To delete Ssrp1 cells were treated with hydroxytamoxifen for 5 days.
Contributor(s) Gurova K, Wang J
Citation(s) 32498018
Submission date Jun 13, 2019
Last update date Jul 14, 2021
Contact name Katerina Gurova
Phone 716-845-4760
Organization name Roswell Park Cancer Institute
Department Cell Stress Biology
Street address Elm and Carlton Str
City Buffalo
State/province NY
ZIP/Postal code 14127
Country USA
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (38)
GSM3887789 RNA-seq_flox immortalized cells variant#2 control
GSM3887790 RNA-seq_flox immortalized cells variant#3 control
GSM3887791 RNA-seq_flox immortalized cells variant#2 4-OHT
BioProject PRJNA548766
SRA SRP201341

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Supplementary file Size Download File type/resource
GSE132695_CountsSubread.csv.gz 433.3 Kb (ftp)(http) CSV
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